Covid-19 Research

Article Figures

Development of a Fluorescence Isothermal Recombinase Polymerase Amplification Assay for Rapid Detection of Feline Calicivirus

View article figures, article metadata, topics, DOI, abstract, and related articles from the JBRES article record.

View Abstract Article Library
Article Type: Research Article Published: 2025-08-25 Volume/Issue: 6 / 8 Pages: 1088-1095

Development of a Fluorescence Isothermal Recombinase Polymerase Amplification Assay for Rapid Detection of Feline Calicivirus

Yujun Zhu, Shuzhou Huang, Bihong Huang, Yuexiao Lian, Tongyuan Zhang, Feng Cong*, and Miaoli Wu*
Abstract FullText HTML PDF DOI
Veterinary Science Biology Virology
Development of a Fluorescence Isothermal Recombinase Polymerase Amplification Assay for Rapid Detection of Feline Calicivirus
jbres2163-g001.webp

Article Figures

Abstract

<p>Feline Calicivirus (FCV) is responsible for a highly contagious disease in domestic cats. FCV may cause multiple symptoms and even death to the infected cats. A simple and cost-effective real-time RPA assay was developed for rapid detection of FCV in clinical samples. In this study, specific primers and probe were designed from the genome of FCV that prevalent in south China. The real-time RPA assay was carried out at 39℃ for 20min before signal analysis by the fluorescence detector. The specificity and sensitivity were thoroughly validated and the results showed that no cross-reaction with irrelevant pathogens were found during the amplification, indicating the good specificity of the new developed real-time RPA assay. RNA standards were constructed and diluted to evaluate the limit of detection. The results showed that the detection limit of the real-time RPA assay could achieve 100 copies/μl, suggesting the high sensitivity of the assay. Additionally, the real-time RPA assay showed excellent performance in clinical sample detection, when compared with a TaqMan qPCR assay. The detection rate of FCV was 38.5% (57/148) for real-time RPA assay and it was a little higher than 37.2% (55/148) of the qPCR assay. Taking all together, the real-time RPA assay had potential application of FCV detection in clinical diagnosis. In conclusion, the new developed real-time RPA assay has provided an alternative strategy for rapid and sensitive detection of FCV in laboratories and animal clinics, especially those with limited facilities.<br></p>

Similar Topic Articles

  • Prevalence and Molecular Confirmation of Corynebacterium pseudotuberculosis in Sheep Populations of Argentine Patagonia: Implications for Wildlife Conservation and One Health
    Diaz Lucia M*, Cortes Carla A, Pujana, Matias, Alvarez, Laura A, Randazzo Viviana R and Gallardo, Adriana A
    Published: 2026-03-27
    Abstract PDF DOI
  • Tail Biting in Pigs Behavioral Signs Welfare Impact Risk Factors and Prevention Strategies
    Ishaya UG*, Vanessa AM, Yolanda D, Rutendo PM, Methun CD, Abdullateef AI, Chidiebere NA, Michael Idowu B and Samuel UE
    Published: 2025-09-20
    Abstract FullText HTML PDF DOI
  • A Mutational Pathway Leading to Thyroid Tumors During Medication for Feline Hyperthyroidism
    Ryunosuke Kikuchi1,3*, Yumiko Kagawa2 and Rosario Placido Roberto da Costa3
    Published: 2025-07-28
    Abstract FullText HTML PDF DOI
  • Valuing the Relative Importance of Goat Farmers Trait Preferences: Implication for Designing Community Based Breeding Program
    Kefelegn Kebede* and Ahmed Usman
    Published: 2023-10-11
    Abstract FullText HTML PDF DOI
Publish with JBRES — Peer-reviewed, multidisciplinary Open Access with rapid review, DOI, and global visibility.
Double-Blind CrossRef DOI Discoverable
Submit Manuscript Membership