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New Spectrophotometric and Electrochemical Enzyme Biosystem Based on Laccase and Ionic Liquid for the Detection of Glyphosate in Biological Samples

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Article Type: Research Article Published: 2025-10-30 Volume/Issue: 6 / 10 Pages: 1614-1625

New Spectrophotometric and Electrochemical Enzyme Biosystem Based on Laccase and Ionic Liquid for the Detection of Glyphosate in Biological Samples

Galai S, HajKacem S, Aladnani A, Rosatella A, de Los Rios AP, Fernandez FJH, Omar S, Afonso C, Diouani MF
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Biochemistry Bioengineering Toxicology
New Spectrophotometric and Electrochemical Enzyme Biosystem Based on Laccase and Ionic Liquid for the Detection of Glyphosate in Biological Samples
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Abstract

<p>Glyphosate (N- (Phosphonomethyl) glycine), one of the most widely used pesticides in the world. The detection methods are difficult to implement, time consuming and expensive due to its chemical properties and its low prevalence; there is a strong need to develop quick-sensitive analytical methods for Glyphosate (GLP) assay and monitoring. For this purpose, a new biosystem based on enzyme reaction was implemented by laccase, redox mediator (Acetosyringone: ASGN), and ionic liquid (IL, as conservator and activator) to catalyse GLP. The laccase-catalytic system has been investigated by two analytical methods: spectrophotometric and electrochemical one. In terms of efficiency, the detection limit for spectrophotometric method was 25 μM GLP, while electrochemical method was even lowest around 5 μM GLP. The developing biosensor based on this enzymatic system has been carried out using gold-plated screen-printed electrode and Nafion polymer for laccase, redox mediator and ionic liquid complex immobilization. GLP samples were successfully analyzed using Cyclic Voltammetry (CV) measurement at scan rate of 100 mV/s. The concentration of GLP was accurately determined in the range of 5 μM to 15 μM GLP, and high correlation rate (98%) between current density and GLP concentration was determined using the laccase-based-biosensor, which shown good reproducibility and repeatability, high selectivity and therefore it has been used for GLP assays in biological samples (Cell lysate and culture medium).<br></p>

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