Covid-19 Research

Research Article

Evaluation of Direct and Indirect Antioxidant Properties of Selected Four Natural Chemical Compounds: Quercetin, Epigallocatechin-3-Gallate, Indole-3-Carbinol and Sulforaphane by DPPH Radical Scavenging Assay

Journal of Biomedical Research & Environmental Sciences article abstract with citation details, DOI, publication dates, subject areas, full text links, and references.

View DOI Submit Manuscript

Article Details

Publication record, authors, dates, abstract, and full text access.

Open Access
Article Type Research Article
Subject General Science
OCLC 8873203482
Maha J Hashim* and Jeffrey R Fry
Issue: Volume1-Issue8
Pages: 389-392
Received: 2020-12-03
Accepted: 2020-12-14
Published: 2020-12-15

Abstract

The main characteristic of antioxidants is the capacity to scavenge free radicals produced during cell metabolism, and thus they prevent oxidative stress, which may reduce the risk of many diseases. In this study, we evaluate the antioxidant properties of selected four compounds Quercetin (Q), Epigallocatechin-3-Gallate, (EGCG), Indole-3-Carbinol (I3C) and Sulforaphane (SF) by DPPH assay. The view is to establish the distinction between direct and indirect antioxidants, which would be the form of the basis for subsequent cellular antioxidant assays in our further studies. For sample assay: 20 μL of antioxidant solutions of Q, EGCG, I3C, and SF was added to 180 of 2,2- Diphenyl-1-Picrylhydrazyl (DPPH) solution. For blank solution, DMSO was used. Leaving the plate for 15 min in a dark place and measure the absorbance at 540 nm. The results demonstrated that Q and EGCG possess direct antioxidant properties, which can be used in further cellular studies. I3C and SFN did not appear to possess any direct antioxidant behaviours during DPPH radical scavenging.

FullText HTML FullText PDF DOI: 10.37871/jbres1170 Crossmark

Certificate of Publication

Certificate of Publication

Copyright

© 2020 Hashim MJ, et al. Distributed under Creative Commons CC-BY 4.0 Creative CommonsAttribution

How to cite this article

Hashim MJ, Fry JR. Evaluation of Direct and Indirect Antioxidant Properties of Selected Four Natural Chemical Compounds: Quercetin, Epigallocatechin-3-Gallate, Indole-3-Carbinol and Sulforaphane by DPPH Radical Scavenging Assay. J Biomed Res Environ Sci. 2020 Dec 15; 1(8): 389-392. doi: 10.37871/jbres1170 Article ID: jbres1170

Subject area(s)

Material Science

References

  1. Reichard P, Ehrenberg A. Ribonucleotide reductase--a radical enzyme. Science. 1983 Aug 5;221(4610):514-9. doi: 10.1126/science.6306767. PMID: 6306767.
  2. Lima CF, Valentao PC, Andrade PB, Seabra RM, Fernandes-Ferreira M, Pereira-Wilson C. Water and methanolic extracts of Salvia officinalis protect HepG2 cells from t-BHP induced oxidative damage. Chem Biol Interact. 2007 Apr 25;167(2):107-15. doi: 10.1016/j.cbi.2007.01.020. Epub 2007 Feb 12. PMID: 17349617.
  3. Agudo A, Cabrera L, Amiano P, Ardanaz E, Barricarte A, Berenguer T, Chirlaque MD, Dorronsoro M, Jakszyn P, Larrañaga N, Martínez C, Navarro C, Quirós JR, Sánchez MJ, Tormo MJ, González CA. Fruit and vegetable intakes, dietary antioxidant nutrients, and total mortality in Spanish adults: findings from the Spanish cohort of the European Prospective Investigation into Cancer and Nutrition (EPIC-Spain). Am J Clin Nutr. 2007 Jun;85(6):1634-42. doi: 10.1093/ajcn/85.6.1634. Erratum in: Am J Clin Nutr. 2008 Oct;88(4):1181. PMID: 17556703.
  4. Barrera LN, Cassidy A, Johnson IT, Bao Y, Belshaw NJ. Epigenetic and antioxidant effects of dietary isothiocyanates and selenium: potential implications for cancer chemoprevention. Proc Nutr Soc. 2012 May;71(2):237-45. doi: 10.1017/S002966511200016X. Epub 2012 Mar 6. PMID: 22391025.
  5. Riboli E, Norat T. Epidemiologic evidence of the protective effect of fruit and vegetables on cancer risk. Am J Clin Nutr. 2003 Sep;78(3 Suppl):559S-569S. doi: 10.1093/ajcn/78.3.559S. PMID: 12936950.
  6. Zafra-Stone S, Yasmin T, Bagchi M, Chatterjee A, Vinson JA, Bagchi D. Berry anthocyanins as novel antioxidants in human health and disease prevention. Mol Nutr Food Res. 2007 Jun;51(6):675-83. doi: 10.1002/mnfr.200700002. PMID: 17533652.
  7. Huang D, Ou B, Prior RL. The chemistry behind antioxidant capacity assays. J Agric Food Chem. 2005 Mar 23;53(6):1841-56. doi: 10.1021/jf030723c. PMID: 15769103.
  8. Clarke G, Ting KN, Wiart C, Fry J. High Correlation of 2,2-diphenyl-1-picrylhydrazyl (DPPH) Radical Scavenging, Ferric Reducing Activity Potential and Total Phenolics Content Indicates Redundancy in Use of All Three Assays to Screen for Antioxidant Activity of Extracts of Plants from the Malaysian Rainforest. Antioxidants (Basel). 2013 Jan 4;2(1):1-10. doi: 10.3390/antiox2010001. PMID: 26787618; PMCID: PMC4665400.
  9. BLOIS M. Antioxidant Determinations by the Use of a Stable Free Radical. Nature. 1958 April 181;1199-1200.
  10. https://go.nature.com/3ngcBi8
  11. Mishra K, Ojha H, Chaudhury NK. Estimation of antiradical properties of antioxidants using DPPH center dot assay: A critical review and results. Food Chemistry. 2012;130(4):1036-1043 doi: 10.1016/j.foodchem.2011.07.127
  12. Nara K, Miyoshi T, Honma T, Koga H. Antioxidative activity of bound-form phenolics in potato peel. Biosci Biotechnol Biochem. 2006 Jun;70(6):1489-91. doi: 10.1271/bbb.50552. PMID: 16794331.
  13. Stanner SA, Hughes J, Kelly CN, Buttriss J. A review of the epidemiological evidence for the ‘antioxidant hypothesis’. Public Health Nutr. 2004 May;7(3):407-22. doi: 10.1079/phn2003543. PMID: 15153272.
  14. Xiao J, Kai G. A review of dietary polyphenol-plasma protein interactions: characterization, influence on the bioactivity, and structure-affinity relationship. Crit Rev Food Sci Nutr. 2012;52(1):85-101. doi: 10.1080/10408398.2010.499017. PMID: 21991992.
  15. Maha J Hashim, Jeffrey R Fry. Influence of Extracellular Protein on the Cytoprotective Effects of Two Model Phytochemicals. Mol Biol. 2019;8(1). doi: 10.4172/2168-9547.1000227
  16. Wagner C, Fachinetto R, Dalla Corte CL, Brito VB, Severo D, de Oliveira Costa Dias G, Morel AF, Nogueira CW, Rocha JB. Quercitrin, a glycoside form of quercetin, prevents lipid peroxidation in vitro. Brain Res. 2006 Aug 30;1107(1):192-8. doi: 10.1016/j.brainres.2006.05.084. Epub 2006 Jul 7. PMID: 16828712.
  17. Chiodo SG, Leopoldini M, Russo N, Toscano M. The inactivation of lipid peroxide radical by quercetin. A theoretical insight. Phys Chem Chem Phys. 2010 Jul 21;12(27):7662-70. doi: 10.1039/b924521a. Epub 2010 Mar 29. PMID: 20596589.
  18. RiceEvans C, Miller N. Measurement of the antioxidant status of dietary constituents, low density lipoproteins and plasma. Prostaglandins Leukotrienes and Essential Fatty Acids. 1997;57:499-505.
  19. https://bit.ly/3mhFTLG
  20. Trouillas P, Marsal P, Siri D, Lazzaroni R, Duroux JL. A DFT study of the reactivity of OH groups in quercetin and taxifolin antioxidants: The specificity of the 3-OH site. Food Chemistry. 2006;97:679-688.
  21. https://bit.ly/2JT3DJi
  22. Rice-Evans CA, Miller NJ, Paganga G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med. 1996;20(7):933-56. doi: 10.1016/0891-5849(95)02227-9. Erratum in: Free Radic Biol Med 1996;21(3):417. PMID: 8743980.
  23. Salah N, Miller NJ, Paganga G, Tijburg L, Bolwell GP, Rice-Evans C. Polyphenolic flavanols as scavengers of aqueous phase radicals and as chain-breaking antioxidants. Arch Biochem Biophys. 1995 Oct 1;322(2):339-46. doi: 10.1006/abbi.1995.1473. PMID: 7574706.
Publish with JBRES — Peer-reviewed, multidisciplinary Open Access with rapid review, DOI, and global visibility.
Double-Blind CrossRef DOI Discoverable
Submit Manuscript Membership