Evaluation of Direct and Indirect Antioxidant Properties of Selected Four Natural Chemical Compounds: Quercetin, Epigallocatechin-3-Gallate, Indole-3-Carbinol and Sulforaphane by DPPH Radical Scavenging Assay

Maha J Hashim*; Jeffrey R Fry

**Maha J Hashim* and Jeffrey R Fry**

Volume1-Issue8
Dates: Received: 2020-12-03 | Accepted: 2020-12-14 | Published: 2020-12-15
Pages: 389-392

Abstract

The main characteristic of antioxidants is the capacity to scavenge free radicals produced during cell metabolism, and thus they prevent oxidative stress, which may reduce the risk of many diseases. In this study, we evaluate the antioxidant properties of selected four compounds Quercetin (Q), Epigallocatechin-3-Gallate, (EGCG), Indole-3-Carbinol (I3C) and Sulforaphane (SF) by DPPH assay. The view is to establish the distinction between direct and indirect antioxidants, which would be the form of the basis for subsequent cellular antioxidant assays in our further studies. For sample assay: 20 μL of antioxidant solutions of Q, EGCG, I3C, and SF was added to 180 of 2,2- Diphenyl-1-Picrylhydrazyl (DPPH) solution. For blank solution, DMSO was used. Leaving the plate for 15 min in a dark place and measure the absorbance at 540 nm. The results demonstrated that Q and EGCG possess direct antioxidant properties, which can be used in further cellular studies. I3C and SFN did not appear to possess any direct antioxidant behaviours during DPPH radical scavenging.

FullText HTML FullText PDF DOI: 10.37871/jbres1170

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Hashim MJ, Fry JR. Evaluation of Direct and Indirect Antioxidant Properties of Selected Four Natural Chemical Compounds: Quercetin, Epigallocatechin-3-Gallate, Indole-3-Carbinol and Sulforaphane by DPPH Radical Scavenging Assay. J Biomed Res Environ Sci. 2020 Dec 15; 1(8): 389-392. doi: 10.37871/jbres1170 Article ID: jbres1170

Subject area(s)

Material Science

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