Raffaele Ansovini* and Leonardo Compagnucci
Volume1-Issue2
Dates: Received: 2020-05-19 | Accepted: 2020-06-15 | Published: 2020-06-17
Pages: 024-038
Abstract
There are several viruses that are not currently covered by efficient antiviral terapies, as towards Poliovirus and the new Covid19. The spread of atypical viral strains strongly raises the need of having efficient terapies with wide coverage against viruses.
In the following experiments it was used a composition comprising Glutathione Reductase (GSSG-r) and Oxidized Glutathione (GSSG), which we call GR compound, for pharmaceutical use as antiviral, in vitro, towards RNA nad DNA virus, and in vivo towards a Swine Flu Virus (H1N1) adapted to the mouse.
Glutathione is present in the cells in form of Reduced Glutathyione (GSH) and Oxidized Glutathyione (GSSG). The GSH:GSSG ratio indicates the antioxidant capacity of the cell and it is maintained in favour of GSH due to GSSG-r, an enzyme belonging to the class of oxidoreductase which rigenerates GSH starting from GSSG.
The GR compound binds to the membrane of cells infected with virus, and repolarizes the membranes so that the Glutamate comes back in the cell and the synthesis of Glutathione starts again. Maintaining a correct GSH:GSSG ratio is an essential requirement for the vitality and survival of the cell. This technology exerts its primary action electrochemically (oxidation-reductive state) both in the host cell and in the virus. Practically the virus, entering the cell, depolarises the cell membrane thus preventing the entry of Glutamate (due to the inhibition of its transport carrier), which is fundamental for the synthesis of Glutathione.
Glutathione continuosly passing from reduced to oxidized generates and maintains the reductive-oxide state, necessary for the cell; all this leads to the non-formation of the sulfur bridges of viral proteins, which thus, changed in allosterism and spatial configuration, are completely eliminated by the immune system; all viruses are weakened, HIV, Covid19, Polio, Ebola, because all viruses must go through this step: the protein formation.
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DOI: 10.37871/jels1117
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© 2020 Ansovini R, et al. Distributed under Creative Commons CC-BY 4.0 
How to cite this article
Ansovini R. Determination of an Antiviral Activity of a Composition Comprising Glutathione Reductase (GSSG-R) and Oxidized Glutathione (GSSG) for Pharmaceutical use: Experiments In vitro and In vivo. J Biomed Res Environ Sci. 2020 Jun 17; 1(2): 029-038. doi: 10.37871/jels1117, Article ID: jels1117
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References
- Davis GL. Treatment of chronic Hepatitis C; British Medical Journal. 2001; 323: 141-142. DOI: https://doi.org/10.1136/bmj.323.7322.1141
- Donovan DJO, Katkin JP, Tamura T, Smith CV, Welty SE. Attenuation of hyperoxia-induced growth inhibition in H441 cells by gene transfer of mitochondrially targeted glutathione reductase. Am J Respir Cell Mol Biol. 2000; 22: 732-738. DOI: https://doi.org/10.1165/ajrcmb.22.6.3836
- Mylonakis E, Maria P, Josiah DR. Plasma viral load testing in the management of HIV infection. American Family Physician. 2001; 63: 483-490. https://bit.ly/37BaheE
- Wong KK, Vanoni MA, Blanchard JS. Glutathione Reductase: Solvent equilibrium and kinetic isotope effects. Biochemistry. 1988; 27; 7091-7096. DOI: https://doi.org/10.1021/bi00418a063
- Mustafa E, Hülya D, Halis S. Purification of glutathione reductase from chicken liver and investigation of kinetic properties. Applied Biochemistry and Biotechnology. 2005; 125: 127-138. DOI: https://doi.org/10.1385/abab:125:2:127
- Garacia E, Palamaraa AT, Francescoa Pdi, Favallia C, Ciriolo MR, Rotilio G. Glutathione inhibits replication and expression of viral proteins in cultured cells infected with sendai virus. Biochemical and Biophysical Research Communications. 1992; 188: 1090-1096. DOI: https://doi.org/10.1016/0006-291X(92)91343-O
- V Boggaram, K Larson, B Mannervik. Characterization of glutathione reductase from porcine erythrocytes. Biochim Biophys Acta. 1978; 527: 337-347. DOI: https://doi.org/10.1016/0005-2744(78)90348-0
- Anonymous, Glutathione Reductase Assay Kit, Catalog# 7510-100-K, Trevigen. 2008; 1-7.
- Schinazi RF, Ilan E, Black PL, Yao X, Dagan S. Cell-based and animal models for Hepatitis B and C viruses. Antivir Chem Chemother. 1999; 10: 99-114.
