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Extraction and Antioxidant Activity of Polysaccharides from Tremella fuciformis by Deep Eutectic Solvents

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Open Access
Article Type Research Article
Subject Biology Group
OCLC JBRES Record
Hua Zhang, Yashu Wei, Xingjian Ma, Yan He and Xia Ma
Issue: Volume7-Issue6
Pages: 1-18
Received: 2026-06-16
Accepted: 2026-06-26
Published: 2026-06-29

Abstract

Tremella fuciformis polysaccharidess possess broad application potential in diverse industrial fields. Submerged fermentation is an efficient technology for producing fermented Tremella fuciformis polysaccharides FTP. In this study, Deep Eutectic Solvents (DES) were adopted to extract FTP from fermentation broths after comparing multiple extraction strategies. The structural characteristics, antioxidant activity, and viscosity stability of the purified components were systematically characterized. Extraction parameters were optimized via Box–Behnken design of response surface methodology. Under optimal conditions, the FTP extraction yield reached 75.35 mg/g, representing an 88.06% improvement relative to the traditional hot water extraction and ethanol precipitation. The characterization results of purified components (FTP1 and FTP2) revealed the presence of ?-pyranoside bonds and a triple-helix conformations in both compounds. Assays for antioxidant activities demonstrated that FTP1 exhibited significantly greater DPPH and hydroxyl radical scavenging activities compared to FTP2. The results of the viscosity stability test indicate that both FTP1 and FTP2 are suitable for various applications in the food industry. Notably, this is the first report employing deep eutectic solvents (DES) to extract Tremella fuciformis polysaccharidess (FTP) from Tremella fuciformis fermentation broth. This study systematically validates the comprehensive advantages of DES against conventional extraction techniques and offers a sustainable green protocol for the preparation of polysaccharidess derived from fungal submerged fermentation. Collectively, FTP1 and FTP2 hold great industrial development value as natural functional biomaterials.

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© 2026 Zhang H. Distributed under Creative Commons CC-BY 4.0 Creative CommonsAttribution

How to cite this article

Zhang H, Wei Y, Ma X, He Y, Ma X. Extraction and Antioxidant Activity of Polysaccharides from Tremella fuciformis by Deep Eutectic Solvents. J Biomed Res Environ Sci. 2026 June 29; 7(6): 18. Doi: 10.37872/jbres2309

Subject area(s)

BiochemistryBiotechnology

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