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Detection of Human Cytomegalovirus DNA in Plasma from Patients with Brain Tumors Using a Sensitive qPCR Assay

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Open Access
Article Type Clinical Trials
Subject Medicine Group
OCLC JBRES Record
Inti Peredo H, Yaiw KC, Bartek J, Rahbar A and Su00f6derberg-Naucler C
Issue: Volume6-Issue9
Pages: 1353-1362
Received: 2025-09-06
Accepted: 2025-09-20
Published: 2025-09-23

Abstract

Background: Human Cytomegalovirus (HCMV) has been detected in tissue samples from patients with glioblastoma, but its presence in blood has been less studied.
Methods: In this prospective study, we analyzed the levels of HCMV-DNA in 59 plasma samples obtained from patients with brain tumors and from 114 healthy blood donors as controls. Detection was performed using a sensitive qPCR assay.
Results: HCMV-IE DNA was detected in plasma samples from patients with glioblastoma (n = 18), astrocytoma grades II-III (n = 11), and brain metastasis (n = 8). These groups showed significantly lower ΔCT values than healthy donors (p < 0.0001 for glioblastoma and astrocytoma; p = 0.0007 for brain metastases), consistent with higher circulating viral DNA levels than in controls. No significant difference was observed between patients with secondary glioblastoma and healthy donors.
Conclusions: HCMV-DNA is present at higher levels in plasma from patients with glioblastoma, astrocytoma grades II–III, and brain metastases compared with healthy individuals. Quantification of HCMV DNA in plasma by qPCR is a reliable approach for detecting HCMV in brain tumour patients.

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© 2025 Inti Peredo H. Distributed under Creative Commons CC-BY 4.0 Creative CommonsAttribution

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Inti Peredo H, Yaiw KC, Bartek J, Rahbar A and Söderberg-Naucler C. Detection of Human Cytomegalovirus DNA in Plasma from Patients with Brain Tumors Using a Sensitive qPCR Assay. J Biomed Res Environ Sci. 2025 Sept 23; 6(9): 1353- 1362. doi: 10.37871/jbres2190, Article ID: JBRES2190, Available at: https://www.jelsciences.com/articles/jbres2190.pdf

Subject area(s)

Brain DisordersBrain Tumors

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