Alpha-Chymotripsin Immobilization on Amino-Functionalized ZnO Nanoparticles to Prepare ZnO/PMMA Nanocomposites Coatings to Create Antimicrobial and Hydrophobic Surfaces on Cotton Fabric

Sorna Gowri Vijaya Kumar*; u00a0Hideaki Morikawa; u00a0Kim Ick Soo; Gopiraman Mayakrishnan; Jyoti Lodhi; Raju Khan

**Sorna Gowri Vijaya Kumar*,u00a0Hideaki Morikawa,u00a0Kim Ick Soo, Gopiraman Mayakrishnan, Jyoti Lodhi and Raju Khan**

Volume6-Issue5
Dates: Received: 2025-03-28 | Accepted: 2025-05-08 | Published: 2025-05-09
Pages: 407-416

Abstract

Incorporation of enzyme immobilized ZnO nanoparticles in to hydrophilic polymer Polymethyl metharcylate (PMMA) has been considered in this present study with the goal of developing antimicrobial hydrophobic textile coatings. The enzyme immobilized nanoparticles polymer coatings on textiles by dip coating, using the polymer Polymethyl methacrylate and proteolytic enzymes alpha chymotripsin immobilized green synthesized, amino functionalized ZnO nanoparticles. Immobilization of enzymes on nanoparticles with large surface to volume ratio provides obvious advantage. Alpha chymotripsin was immobilized on amino functionalized ZnO nanoparticles dispersed in n-proponal. The enzyme immobilized ZnO nanoparticles were used for making the ZnO/PMMA polymer nanocomposite coating. This coating was applied on cotton textile by dip coating and the coated textiles were evaluated for antimicrobial and hydrophobicity Properties.

FullText HTML FullText PDF DOI: 10.37871/jbres2098

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How to cite this article

Vijaya Kumar SG,􀉸Morikawa H,􀉸Soo KI, Mayakrishnan G, Lodhi J, Khan R. Alpha-Chymotripsin Immobilization on Amino-Functionalized ZnO Nanoparticles to Prepare ZnO/PMMA Nanocomposites Coatings to Create Antimicrobial and Hydrophobic Surfaces on Cotton Fabric. J Biomed Res Environ Sci. 2025 May 09; 6(5): 407-416. doi: 10.37871/jbres2098, Article ID: JBRES2098, Available at: https://www.jelsciences.com/articles/jbres2098.pdf

Subject area(s)

Nanotechnology

References

SI Shahid-ul-Islam, BS Butola and F Mohammad. Silver nanomaterials as future colorants and potential antimicrobial agents for natural and synthetic textile materials. RSC. 2016;6: 44232-44247. doi: 10.1039/C6RA05799C.
Jeremiah SS, Miyakawa K, Morita T, Yamaoka Y, Ryo A. Potent antiviral effect of silver nanoparticles on SARS-CoV-2. Biochem Biophys Res Commun. 2020 Nov 26;533(1):195-200. doi: 10.1016/j.bbrc.2020.09.018. Epub 2020 Sep 11. PMID: 32958250; PMCID: PMC7486059.
Román LE, Gomez ED, Solís JL, Gómez MM. Antibacterial Cotton Fabric Functionalized with Copper Oxide Nanoparticles. Molecules. 2020 Dec 9;25(24):5802. doi: 10.3390/molecules25245802. PMID: 33316935; PMCID: PMC7764683.
PSadalage PS, Nimbalkar MS, Sharma KKK, Patil PS, Pawar KD. Sustainable approach to almond skin mediated synthesis of tunable selenium microstructures for coating cotton fabric to impart specific antibacterial activity. J Colloid Interface Sci. 2020 Jun 1;569:346-357. doi: 10.1016/j.jcis.2020.02.094. Epub 2020 Feb 25. PMID: 32126347.
M Zayed, M Bakr, H Ghazal. Recent developments in the utilization of polymer nanocomposites in textile applications. Journal of Textiles, Coloration and Polymer Science. 2023;20:147-170. doi: 10.21608/jtcps.2023.193744.1172.
Thallinger B, Prasetyo EN, Nyanhongo GS, Guebitz GM. Antimicrobial enzymes: an emerging strategy to fight microbes and microbial biofilms. Biotechnol J. 2013 Jan;8(1):97-109. doi: 10.1002/biot.201200313. PMID: 23281326.
Olsen SM, Pedersen LT, Laursen MH, Kiil S, Dam-Johansen K. Enzyme-based antifouling coatings: a review. Biofouling. 2007;23(5-6):369-83. doi: 10.1080/08927010701566384. PMID: 17852071.
Pettitt ME, Henry SL, Callow ME, Callow JA, Clare AS. Activity of commercial enzymes on settlement and adhesion of cypris larvae of the barnacle Balanus amphitrite, spores of the green alga Ulva linza, and the diatom Navicula perminuta. Biofouling. 2004 Dec;20(6):299-311. doi: 10.1080/08927010400027068. PMID: 15804714.
Aldred N, Phang IY, Conlan SL, Clare AS, Vancso GJ. The effects of a serine protease, Alcalase, on the adhesives of barnacle cyprids (Balanus amphitrite). Biofouling. 2008;24(2):97-107. doi: 10.1080/08927010801885908. PMID: 18231899.
Caro A, Humblot V, Méthivier C, Minier M, Salmain M, Pradier CM. Grafting of lysozyme and/or poly(ethylene glycol) to prevent biofilm growth on stainless steel surfaces. J Phys Chem B. 2009 Feb 19;113(7):2101-9. doi: 10.1021/jp805284s. PMID: 19166331.
NA Ibrahim, M Gouda, AM Elshafei, OM Abdel-Fatah. Antimicrobial activity of cotton fabrics containing immobilized enzymes. Journal of Applied Polymer Science. 2007;104:1754-1761. doi: 10.1002/app.25821.
Cadnum JL, Li DF, Redmond SN, John AR, Pearlmutter B, Donskey CJ. Effectiveness of Ultraviolet-C Light and a High-Level Disinfection Cabinet for Decontamination of N95 Respirators. Pathog Immun. 2020 Apr 20;5(1):52-67. doi: 10.20411/pai.v5i1.372. PMID: 32363254; PMCID: PMC7192214.
Rowan NJ, Laffey JG. Challenges and solutions for addressing critical shortage of supply chain for personal and protective equipment (PPE) arising from Coronavirus disease (COVID19) pandemic - Case study from the Republic of Ireland. Sci Total Environ. 2020 Jul 10;725:138532. doi: 10.1016/j.scitotenv.2020.138532. Epub 2020 Apr 6. PMID: 32304970; PMCID: PMC7195029.
Derraik JGB, Anderson WA, Connelly EA, Anderson YC. Rapid Review of SARS-CoV-1 and SARS-CoV-2 Viability, Susceptibility to Treatment, and the Disinfection and Reuse of PPE, Particularly Filtering Facepiece Respirators. Int J Environ Res Public Health. 2020 Aug 22;17(17):6117. doi: 10.3390/ijerph17176117. PMID: 32842655; PMCID: PMC7504573.
CB Hiragond AS Kshirsagar, VV Dhapte, T Khanna, P Joshi, PV. More, enhanced anti-microbial response of commercial face mask using colloidal silver nanoparticles. Vacuum. 2018;156:475-482. doi: 10.1016/j.vacuum.2018.08.007.
Mokhena TC, Luyt AS. Electrospun alginate nanofibres impregnated with silver nanoparticles: Preparation, morphology and antibacterial properties. Carbohydr Polym. 2017 Jun 1;165:304-312. doi: 10.1016/j.carbpol.2017.02.068. Epub 2017 Feb 21. PMID: 28363554.
F Hui and C Debiemme-Chouvy. Antimicrobial N-Halamine polymers and coatings: A review of their synthesis, characterization, and applications. Biomacromolecules. 2013;14:585-601. doi: 10.1021/bm301980q.
J Choi, SG Ryu, SGB Lee, WB Ying, KJ Lee, N-chloro hydantoin functionalized polyurethane fibers toward protective cloth against chemical warfare agents. Polymer. 138(2018) 146-155. doi: 10.1016/j.polymer.2018.01.066.
P Tang, M Zhang, B Ji, T Yong, G Sun. Hierarchical nucleophilic nanofibrous membranes for fast, durable, and bare-eye visible detoxification of carcinogenic alkylating toxicants. Adv Funct Mater. 2019;29:1905990. doi: 10.1002/adfm.201905990.
Demir B, Cerkez I, Worley SD, Broughton RM, Huang TS. N-Halamine-modified antimicrobial polypropylene nonwoven fabrics for use against airborne bacteria. ACS Appl Mater Interfaces. 2015 Jan 28;7(3):1752-7. doi: 10.1021/am507329m. Epub 2015 Jan 14. PMID: 25587845.
Ghayempour S, Montazer M. Micro/nanoencapsulation of essential oils and fragrances: Focus on perfumed, antimicrobial, mosquito-repellent and medical textiles. J Microencapsul. 2016 Sep;33(6):497-510. doi: 10.1080/02652048.2016.1216187. Epub 2016 Oct 4. PMID: 27701985.
N Kamrudi, S Akbari, M Haghighat Kish. Enhanced control release of thyme essential oils from electrospun nanofiber/polyamidoamine dendritic polymer for antibacterial platforms. Polym Adv Technol. 2020;31:1719-1731. doi: 10.1002/pat.4899.
Kenney PA, Chan BK, Kortright KE, Cintron M, Russi M, Epright J, Lee L, Balcezak TJ, Havill NL, Martinello RA. Hydrogen peroxide vapor decontamination of N95 respirators for reuse. Infect Control Hosp Epidemiol. 2022 Jan;43(1):45-47. doi: 10.1017/ice.2021.48. Epub 2021 Feb 9. PMID: 33557979; PMCID: PMC8185421.
Song P, Zhang X, Wang S, Xu W, Wang F, Fu R, Wei F. Microbial proteases and their applications. Front Microbiol. 2023 Sep 14;14:1236368. doi: 10.3389/fmicb.2023.1236368. PMID: 37779686; PMCID: PMC10537240.
Khafaga DSR, Muteeb G, Elgarawany A, Aatif M, Farhan M, Allam S, Almatar BA, Radwan MG. Green nanobiocatalysts: enhancing enzyme immobilization for industrial and biomedical applications. PeerJ. 2024 Jul 8;12:e17589. doi: 10.7717/peerj.17589. PMID: 38993977; PMCID: PMC11238728.
LS Dyshlyuk, MV Novoselova, TA Rozalenok. Immobilization of chymotrypsin on magnetic Fe3O4 nanoparticles. Foods and Raw Materials. 2013;1:85-88. doi: 10.12737/2060.
Lishchynskyi O, Shymborska Y, Stetsyshyn Y, Raczkowska J, Skirtach AG, Peretiatko T, Budkowski A. Passive antifouling and active self-disinfecting antiviral surfaces. Chem Eng J. 2022 Oct 15;446:137048. doi: 10.1016/j.cej.2022.137048. Epub 2022 May 18. PMID: 35601363; PMCID: PMC9113772.
M Neha, K Vishaka. Application of zinc oxide nano particles using polymeric binders on cotton fabric. Research Journal of Textile and Apparel. 2022;26:310-322. doi: 10.1108/RJTA-02-2021-0018.
MJ Haque, MM Bellah, MR Hassan, S Rahman. Synthesis of ZnO nanoparticles by two different methods & comparison of their structural, antibacterial, photocatalytic and optical properties. Nano Ex. 2020;1:010007. doi: 10.1088/2632-959X/ab7a43.
AA Naser, HZ Al-Sawaad, AS Al-Mubarak. Novel graphene oxide functionalization by urea and thiourea, and their applications as anticorrosive agents for carbon steel alloy in acidic medium. J Mater Environ Sci. 2020;11:404-420.
Mukherjee J, Gupta MN. Alpha chymotrypsin coated clusters of Fe3O4 nanoparticles for biocatalysis in low water media. Chem Cent J. 2012 Nov 8;6(1):133. doi: 10.1186/1752-153X-6-133. PMID: 23137100; PMCID: PMC3505189.
A Kajbafvala, S Zanganeh, E Kajbafvala. Microwave-assisted synthesis of narcis-like Zinc Oxide nanostructures. Journal of Alloys and Compounds. 2010;497:325-329. doi: 10.1016/j.jallcom.2010.03.057.
VS Gowri, L Almeida, MTP Amorim, C Noemia, E Fatima, S Pedro. Functional finishing of polyamide fabrics using ZnO-PMMA nanocomposites. J Mater Sci. 2010;45:2427-2435. doi: 10.1007/s10853-010-4210-4.
JP Jose, J Abraham, H Maria, K Varughese, S Thomas. Contact angle studies in xlpe hybrid nanocomposites with inorganic nanofillers. Macromolecular Symposia. 2016;366:66-78. doi: 10.1002/masy.201650048.