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Exploring the Potential of Xanthatin from Cuban Xanthium strumarium: Isolation, Characterization, and Biological Evaluation

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Open Access
Article Type Research Article
Subject Medicine Group
OCLC JBRES Record
Berenguer A, Osoria L, Gonzalez Suarez N, Manso A, Salomon S, Padron Yaquis AS, Annabi B and Piloto-Ferrer J*
Issue: Volume5-Issue9
Pages: 1176-1186
Received: 2024-09-12
Accepted: 2024-09-20
Published: 2024-09-20

Abstract

Xanthatin possesses anticancer activities through its effect on cancer cell proliferation, apoptosis and metastasis In vitro and In vivo. Here, we described the isolation procedure, characterization and In vitro biological evaluation of the active metabolite xanthatin from Xanthium strumarium grown in Cuba. From the aerial parts of X. strumarium, 995 mg of xanthatin was isolated in the form of prismatic crystals with a yield of 0.07%. The crystals obtained were characterized by HPLC-UV and spectroscopic techniques including UV, IR, and one- and two-dimensional NMR. The signals observed in the spectra obtained confirmed the structure of xanthatin, especially the two-dimensional NMR spectra. The absence of the coupling signal between the H-8 and H-7 protons in the NOESY spectrum allowed us to identify that the fusion of the 7-membered ring and the lactone ring occurs with trans stereochemistry. The isolated xanthatin undergoes a phase transition at 114.30 ± 0.03°C, a value in good agreement with the melting point reported for commercial xanthatin. Biological evaluation showed that xanthatin inhibited the proliferation of HT-29 colorectal cancer cells and HeLa cervical cancer cells, with the greatest inhibitory potential against colorectal cancer. The molecule can also inhibit cell cycle progression by inducing cell cycle arrest at the G2/M phase in HeLa and HT-29 cells, with the greatest effect in HT-29. In conclusion, this study demonstrates promising therapeutic effects of xanthatin from X. strumarium grown in Cuba against colorectal and cervical cancer cell lines, and provides new insights into its potential and effective antitumor properties.

FullText HTML FullText PDF DOI: 10.37871/jbres2006 Crossmark

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© 2024 Berenguer A, et al. Distributed under Creative Commons CC-BY 4.0 Creative CommonsAttribution

How to cite this article

Berenguer A, Osoria L, González Suarez N, Manso A, Salomón S, Padrón Yaquis AS, Annabi B, Piloto-Ferrer J. Exploring the Potential of Xanthatin from Cuban Xanthium strumarium: Isolation, Characterization, and Biological Evaluation. J Biomed Res Environ Sci. 2024 Sept 20; 5(9): 1176-1186. doi: 10.37871/jbres2006, Article ID: JBRES2006, Available at: https://www.jelsciences. com/articles/jbres2006.pdf

Subject area(s)

Pharmacology

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