Daniele Corsaro*
Volume4-Issue5
Dates: Received: 2023-04-24 | Accepted: 2023-05-03 | Published: 2023-05-04
Pages: 849-852
Abstract
During an investigation of amoeba rDNA sequences, a group I intron was identified in the 18S rDNA of Thecamoeba aesculea (Amoebozoa; Discosea) at position S956. Secondary structure of Thecamoeba intron and phylogenetic analysis suggest that it is affiliated with other S956 introns occurring in distantly related amoebae such as acrasid (Percolozoa; Heterolobosea) and myxogastrid (Amoebozoa; Mycetozoa) slime moulds. This is the first report of a nuclear intron occurring within the genus Thecamoeba.
FullText HTML
FullText PDF
DOI: 10.37871/jbres1742
Certificate of Publication

Copyright
© 2023 Corsaro D. Distributed under Creative Commons CC-BY 4.0
How to cite this article
Corsaro D. Characterisation of a Group I Intron in the 18S rDNA of Thecamoeba (Amoebozoa; Discosea). J Biomed Res Environ Sci. 2023 May 04; 4(5): 849-852. doi: 10.37871/jbres1742, Article ID: JBRES1742, Available at: https://www. jelsciences.com/articles/jbres1742.pdf
Subject area(s)
References
- Haugen P, Simon DM, Bhattacharya D. The natural history of group I introns. Trends Genet. 2005 Feb;21(2):111-9. doi: 10.1016/j.tig.2004.12.007. PMID: 15661357.
- Hedberg A, Johansen SD. Nuclear group I introns in self-splicing and beyond. Mob DNA. 2013 Jun 5;4(1):17. doi: 10.1186/1759-8753-4-17. PMID: 23738941; PMCID: PMC3679873.
- Corsaro D, Venditti D. Putative group I introns in the eukaryote nuclear internal transcribed spacers. Curr Genet. 2020 Apr;66(2):373-384. doi: 10.1007/s00294-019-01027-0. Epub 2019 Aug 28. PMID: 31463775.
- Michel F, Westhof E. Modelling of the three-dimensional architecture of group I catalytic introns based on comparative sequence analysis. J Mol Biol. 1990 Dec 5;216(3):585-610. doi: 10.1016/0022-2836(90)90386-Z. PMID: 2258934.
- Suh SO, Jones KG, Blackwell M. A Group I intron in the nuclear small subunit rRNA gene of Cryptendoxyla hypophloia, an ascomycetous fungus: evidence for a new major class of Group I introns. J Mol Evol. 1999 May;48(5):493-500. doi: 10.1007/pl00006493. PMID: 10198116.
- Bhattacarya D, Cannone JJ, Gutell RR. Group I intron lateral transfer between red and brown algal ribosomal RNA. Curr Genet. 2001 Aug;40(1):82-90. doi: 10.1007/s002940100227. PMID: 11570520.
- Simon D, Moline J, Helms G, Friedl T, Bhattacharya D. Divergent histories of rDNA group I introns in the lichen family Physciaceae. J Mol Evol. 2005 Apr;60(4):434-46. doi: 10.1007/s00239-004-0152-2. PMID: 15883879.
- Nandipati SC, Haugli K, Coucheron DH, Haskins EF, Johansen SD. Polyphyletic origin of the genus Physarum (Physarales, Myxomycetes) revealed by nuclear rDNA mini-chromosome analysis and group I intron synapomorphy. BMC Evol Biol. 2012 Aug 31;12:166. doi: 10.1186/1471-2148-12-166. PMID: 22938158; PMCID: PMC3511172.
- Furulund BMN, Karlsen BO, Babiak I, Haugen P, Johansen SD. Structural Organization of S516 Group I Introns in Myxomycetes. Genes (Basel). 2022 May 25;13(6):944. doi: 10.3390/genes13060944. PMID: 35741706; PMCID: PMC9223047.
- Gast RJ, Fuerst PA, Byers TJ. Discovery of group I introns in the nuclear small subunit ribosomal RNA genes of Acanthamoeba. Nucleic Acids Res. 1994 Feb 25;22(4):592-6. doi: 10.1093/nar/22.4.592. PMID: 8127708; PMCID: PMC307848.
- Schroeder-Diedrich JM, Fuerst PA, Byers TJ. Group-I introns with unusual sequences occur at three sites in nuclear 18S rRNA genes of Acanthamoeba lenticulata. Curr Genet. 1998 Jul;34(1):71-8. doi: 10.1007/s002940050368. PMID: 9683678.
- Corsaro D, Köhsler M, Montalbano Di Filippo M, Venditti D, Monno R, Di Cave D, Berrilli F, Walochnik J. Update on Acanthamoeba jacobsi genotype T15, including full-length 18S rDNA molecular phylogeny. Parasitol Res. 2017 Apr;116(4):1273-1284. doi: 10.1007/s00436-017-5406-1. Epub 2017 Feb 11. PMID: 28190156.
- Corsaro D, Venditti D. Nuclear Group I introns with homing endonuclease genes in Acanthamoeba genotype T4. Eur J Protistol. 2018 Oct;66:26-35. doi: 10.1016/j.ejop.2018.07.002. Epub 2018 Jul 18. PMID: 30071371.
- Corsaro D, Köhsler M, Venditti D, Rott MB, Walochnik J. Recovery of an Acanthamoeba strain with two group I introns in the nuclear 18S rRNA gene. Eur J Protistol. 2019 Apr;68:88-98. doi: 10.1016/j.ejop.2019.01.007. Epub 2019 Jan 28. PMID: 30743186.
- Kudryavtsev A, Hausmann K. Thecamoeba aesculea n. sp. (Amoebozoa, Thecamoebidae), a terrestrial amoeba with affinities to Th. sphaeronucleolus (Greeff. 1891). Acta Protozool. 2009 48(2):91-6.
- Smirnov AV, Chao E, Nassonova ES, Cavalier-Smith T. A revised classification of naked lobose amoebae (Amoebozoa: lobosa). Protist. 2011 Oct;162(4):545-70. doi: 10.1016/j.protis.2011.04.004. Epub 2011 Jul 28. PMID: 21798804.
- Corsaro D, Venditti D. Nuclear group I introns in the 18S rDNA subtypes of Vermamoeba vermiformis. Biologia. 2022 Jul ;77(7):1899-1907. doi: 10.1007/s11756-022-01047-1.
- Corsaro D, Walochnik J, Köhsler M, Rott MB. Acanthamoeba misidentification and multiple labels: redefining genotypes T16, T19, and T20 and proposal for Acanthamoeba micheli sp. nov. (genotype T19). Parasitol Res. 2015 Jul;114(7):2481-90. doi: 10.1007/s00436-015-4445-8. Epub 2015 Apr 15. PMID: 25869957.
- Corsaro D, Walochnik J, Venditti D, Hauröder B, Michel R. Solving an old enigma: Morellospora saccamoebae gen. nov., sp. nov. (Rozellomycota), a Sphaerita-like parasite of free-living amoebae. Parasitol Res. 2020 Mar;119(3):925-934. doi: 10.1007/s00436-020-06623-5. Epub 2020 Feb 11. PMID: 32048025.
- Mesentsev YS, Smirnov AV. Thecamoeba cosmophorea n. sp. (Amoebozoa, Discosea, Thecamoebida) - An example of sibling species within the genus Thecamoeba. Eur J Protistol. 2019 Feb;67:132-141. doi: 10.1016/j.ejop.2018.12.003. Epub 2018 Dec 18. PMID: 30616106.
- Mesentsev Y, Smirnov A. Thecamoeba astrologa n. sp. - A new species of the genus Thecamoeba (Amoebozoa, Discosea, Thecamoebida) with an unusually polymorphic nuclear structure. Eur J Protistol. 2021 Oct;81:125837. doi: 10.1016/j.ejop.2021.125837. Epub 2021 Aug 14. PMID: 34583223.