Biostatistical Analysis on Synergetic Therapy of the Three Plants

Bin Zhao*; Xia Jiang

**Bin Zhao* and Xia Jiang**

Volume4-Issue4
Dates: Received: 2023-04-10 | Accepted: 2023-04-19 | Published: 2023-04-21
Pages: 747-759

Abstract

The liver is an important metabolic organ consisting of four different sized lobes in our bodies. The important function of liver includes the elimination of toxic material, detoxication and regulation of the blood, blood clotting, and manufacture of vital hormones. It also plays a crucial role in other activities, such as pathogen protection, synthesis of bile salts, food supply, and blood collecting. There is currently no obvious mechanism that includes direct hepatotoxicity and unfavorable immune response for several study reports on hepatic damage mechanisms. Pharmaceutical bioactivation creates reactive chemical metabolites that interact with lipids, nucleic acid, and proteins in the cell, producing DNA damage, protein malfunction, lipid peroxidation, and oxidative stress. Many illnesses also affect the liver due to its strategic position. Hepatotoxins, due to the overdose of drugs, xenobiotics, alcohol and industrial toxins, can harm the liver. However, Natural and synthetic chemicals are used to treat liver diseases as hepatoprotective agents. This study was conducted to evaluate the hepatoprotective potential of Geranium wallichianum, Elaeagnus parvifolia and Taraxacum officinale against Carbon Tetrachloride (CCl4) induced hepatotoxicity in experimental rats. Extract of all three plants was induced (200 mg/kg) and combination of all three plants were given in (100 mg/kg). Liver enzymes including Alanine Aminotransferase (ALT) and Aspartate Aminotransferase (AST), Alkaline Phosphatase (ALP) and total bilirubin as well as liver histopathology were evaluated to see the effect of herbal treatments. Results of this study suggest that all three plants have hepatoprotective effect and highest effect was noted in synergetic therapy.

FullText HTML FullText PDF DOI: 10.37871/jbres1733

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How to cite this article

Zhao B, Jiang X.Biostatistical Analysis on Synergetic Therapy of the Three Plants. J Biomed Res Environ Sci. 2023 Apr 21; 4(4): 747-759. doi: 10.37871/jbres1733, Article ID: JBRES1733, Available at: https://www.jelsciences.com/articles/ jbres1733.pdf

Subject area(s)

Bioanalysis

References

Maheswari C, Maryammal R, Venkatanarayanan R. Hepatoprotective activity of Orthosiphon stamineus on liver damage caused by paracetamol in rats. Jordan J Biol Sci. 2008 sept;1(3):105-108.
Opoku AR, Ndlovu IM, Terblanche SE, Hutchings A. In vivo hepatoprotective effects of Rhoicissus tridentata subsp. cuneifolia, a traditional Zulu medicinal plant, against CCl4-induced acute liver injury in rats. South African Journal of Botany. 2007;73(3):372-377.
Arhoghro EM, Ekpo EK, Anosike OE, Ibeh OG. Effect of aqueous extract of bitter leaf (Vernonia amygdalina Del) on Carbon tetrachloride (CCl4) induced liver damage in albino Wistar rats. Eur J Sci Res. 2009 January;26(1):12-30.
Soni J, Ansari U, Sharma D, Soni S. Predictive data mining for medical diagnosis: An overview of heart disease prediction. International Journal of Computer Applications. 2011;17:43-48.
Lynch T, Price A. The effect of cytochrome P450 metabolism on drug response, interactions, and adverse effects. Am Fam Physician. 2007 Aug 1;76(3):391-6. PMID: 17708140.
Blazka ME, Wilmer JL, Holladay SD, Wilson RE, Luster MI. Role of proinflammatory cytokines in acetaminophen hepatotoxicity. Toxicol Appl Pharmacol. 1995 Jul;133(1):43-52. doi: 10.1006/taap.1995.1125. PMID: 7597709.
Bourdi M, Masubuchi Y, Reilly TP, Amouzadeh HR, Martin JL, George JW, Shah AG, Pohl LR. Protection against acetaminophen-induced liver injury and lethality by interleukin 10: role of inducible nitric oxide synthase. Hepatology. 2002 Feb;35(2):289-98. doi: 10.1053/jhep.2002.30956. PMID: 11826401.
Polyak SJ, Morishima C, Shuhart MC, Wang CC, Liu Y, Lee DY. Inhibition of T-cell inflammatory cytokines, hepatocyte NF-kappaB signaling, and HCV infection by standardized Silymarin. Gastroenterology. 2007 May;132(5):1925-36. doi: 10.1053/j.gastro.2007.02.038. Epub 2007 Feb 21. PMID: 17484885.
Cornella J, Larrosa I. Decarboxylative carbon-carbon bond-forming transformations of (hetero) aromatic carboxylic acids. Synthesis. 2012;44(5):653-676.
El-Serag HB, Davila JA. Surveillance for hepatocellular carcinoma: in whom and how? Therap Adv Gastroenterol. 2011 Jan;4(1):5-10. doi: 10.1177/1756283X10385964. PMID: 21317990; PMCID: PMC3036965.
Yang JD, Roberts LR. Hepatocellular carcinoma: A global view. Nat Rev Gastroenterol Hepatol. 2010 Aug;7(8):448-58. doi: 10.1038/nrgastro.2010.100. Epub 2010 Jul 13. PMID: 20628345; PMCID: PMC3926946.
Nazeema TH, Brindha V. Antihepatotoxic and antioxidant defense potential of Mimosa pudica. Int J Drug Discov. 2009 December;1(2):1-4. doi: 10.9735/0975-4423.1.2.1-4.
Shepard CW, Finelli L, Alter MJ. Global epidemiology of hepatitis C virus infection. Lancet Infect Dis. 2005 Sep;5(9):558-67. doi: 10.1016/S1473-3099(05)70216-4. PMID: 16122679.
McKillop IH, Moran DM, Jin X, Koniaris LG. Molecular pathogenesis of hepatocellular carcinoma. J Surg Res. 2006 Nov;136(1):125-35. doi: 10.1016/j.jss.2006.04.013. Epub 2006 Oct 3. PMID: 17023002.
Mckillop IH, Moran DM, Jin X, Koniaris LG. Molecular pathogenesis of hepatocellular carcinoma. J Surg Res. 2006 Nov;136(1):125-35. doi: 10.1016/j.jss.2006.04.013. Epub 2006 Oct 3. PMID: 17023002.
World Health Organization (WHO). The world health report 2003: Shaping the future. World Health Organization. 2003.
Pandey S, Seth A, Tiwari R, Singh S, Behl HM, Singh S. Development and evaluation of antimicrobial herbal cosmetic preparation. African Journal of Pharmacy and Pharmacology. 29 May 2014;8(20):514-528. doi: 10.5897/AJPP2013.3967.
Hakemi-Vala M, Makhmor M, Kobarfar F, Kamalinejad M, Heidary M, Khoshnood S. Investigation of antimicrobial effect of Tribulus terrestris L. against some gram positive and negative bacteria and candida spp. Novelty Biomed. 2014 January;2(3):85-90.
Eze JN, Umeora OU, Obuna JA, Egwuatu VE, Ejikeme BN. Cervical cancer awareness and cervical screening uptake at the Mater Misericordiae Hospital, Afikpo, Southeast Nigeria. Ann Afr Med. 2012 Oct-Dec;11(4):238-43. doi: 10.4103/1596-3519.102856. PMID: 23103924.
Bhat CR. The Multiple Discrete-Continuous Extreme Value (MDCEV) model: Role of utility function parameters, identification considerations, and model extensions. Transportation Research Part B: Methodological. 2008;42(3):274-303.
Rasool Hassan BA. Medicinal plants (importance and uses). Pharmaceut Anal Acta. 2012;3(10):2153-2435. doi: 10.4172/2153-2435.1000e139.
Kensa VM, Yasmin SS. Phytochemical screening and antimicrobial activity on Ricinus communis. Plant Sciences Feed. 2011;1:167-173.
Jamil SS, Nizami Q, Salam M. Centella asiatica (Linn.) Urban: A review. Natural product Radiance. 2007;6(2):158-170.
Williamson EM. Synergy and other interactions in phytomedicines. Phytomedicine. 2001 Sep;8(5):401-9. doi: 10.1078/0944-7113-00060. PMID: 11695885.
Tatiya AU, Surana SJ, Sutar MP, Gamit NH. Hepatoprotective effect of poly herbal formulation against various hepatotoxic agents in rats. Pharmacognosy Res. 2012 Jan;4(1):50-6. doi: 10.4103/0974-8490.91040. PMID: 22224062; PMCID: PMC3250040.
Ashoush IS, El-Batawy OI, Gehan A. Antioxidant activity and hepatoprotective effect of pomegranate pell and whey powder in rats. Annals of Agricultural Science. 2013 June;58 (1):27-32.
Asirvatham R, Akhil J. Morinda reticulata Gamble Tubers: A potent hepatoprotective agent against CCl4 and paracetamol induced hepatotoxicity in Wistar albino rats. International Journal of Nature and Life Sciences. 2020 June;4(1):26-39.
Kiran PM, Raju AV, Rao BG. Investigation of hepatoprotective activity of Cyathea gigantea (Wall. ex. Hook.) leaves against paracetamol-induced hepatotoxicity in rats. Asian Pac J Trop Biomed. 2012 May;2(5):352-6. doi: 10.1016/S2221-1691(12)60055-0. PMID: 23569929; PMCID: PMC3609311.
Hurkadale PJ, Shelar PA, Palled SG, Mandavkar YD, Khedkar AS. Hepatoprotective activity of Amorphophallus paeoniifolius tubers against paracetamol induced liver damage in rats. Asian Pac J Trop Biomed. 2012;2(1):238-242.
Venkatachalam U, Muthukrishnan S. Hepatoprotective activity of Desmodium gangeticum in paracetamol induced liver damage in rats. Biomedicine & Preventive Nutrition. 2013 July;3(3):273-277. doi: 10.1016/j.bionut.2012.12.003.
Nurrochmad A, Margono SA, Sardjiman, Hakim AR, Ernawati, Kurniawati E, Fatmawati E. Hepatoprotective and antioxidant activity of pentagamavunon-0 against carbon tetrachloride-induced hepatic injury in rats. Asian Pac J Trop Med. 2013 Jun;6(6):438-42. doi: 10.1016/S1995-7645(13)60070-X. PMID: 23711702.
Li L, Li W, Kim YH, Lee YW. Chlorella vulgaris extract ameliorates carbon tetrachloride-induced acute hepatic injury in mice. Exp Toxicol Pathol. 2013 Jan;65(1-2):73-80. doi: 10.1016/j.etp.2011.06.003. Epub 2011 Jul 8. PMID: 21741806.
Borkham-Kamphorst E, van de Leur E, Zimmermann HW, Karlmark KR, Tihaa L, Haas U, Tacke F, Berger T, Mak TW, Weiskirchen R. Protective effects of lipocalin-2 (LCN2) in acute liver injury suggest a novel function in liver homeostasis. Biochim Biophys Acta. 2013 May;1832(5):660-73. doi: 10.1016/j.bbadis.2013.01.014. Epub 2013 Jan 31. PMID: 23376114.
Saleem M, Ahmed B, Karim M, Ahmed S, Ahmad M, Qadir MI. Hepatoprotective effect of aqueous methanolic extract of Rumex dentatus in paracetamol- induced hepatotoxicity in mice. Bangladesh Journal of Pharmacology. 2014;9(3):284-289.
Chander TR, Reddy YN, Bollikunta V, Warangal. Evaluation of hepatoprotective and antihepatotoxic activity of ethanolic extract of Evolvulus alsinoides Linn on CCl4 induced rats. Asian Journal of Pharmacology and Toxicology. 2014 August;02(04):01-06.
Reddy VR, Kumar V, Reddy MK. Hepatoprotective activity of Elytraria acaulis plant extracts in albino rats. International Journal of Pharmacognosy and Phytochemical Research. 2014 January;6(4):925-929.
Desplaces A, Choppin J, Vogel G, Trost W. The effects of silymarin on experimental phalloidine poisoning. Arzneimittelforschung. 1975 Jan;25(1):89-96. PMID: 125090.
Vogel G, Tuchweber B, Trost W, Mengs U. Protection by silibinin against Amanita phalloides intoxication in beagles. Toxicol Appl Pharmacol. 1984 May;73(3):355-62. doi: 10.1016/0041-008x(84)90087-5. PMID: 6719456.
De Vizcaya-Ruiz A, Barbier O, Ruiz-Ramos R, Cebrian ME. Biomarkers of oxidative stress and damage in human populations exposed to arsenic. Mutat Res. 2009 Mar 31;674(1-2):85-92. doi: 10.1016/j.mrgentox.2008.09.020. Epub 2008 Oct 15. PMID: 18984063.
Akkaya H, Yilmaz O. Antioxidant capacity and radical scavenging activity of Silybum marianum and Ceratonia siliqua. Ekoloji. 2012;21(82):9-16.
Yang CC, Fang JY, Hong TL, Wang TC, Zhou YE, Lin TC. Potential antioxidant properties and hepatoprotective effects of an aqueous extract formula derived from three Chinese medicinal herbs against CCl4-induced liver injury in rats. Int Immunopharmacol. 2013 Jan;15(1):106-13. doi: 10.1016/j.intimp.2012.10.017. Epub 2012 Nov 8. PMID: 23142091.
Yang CC, Fang JY, Hong TL, Wang TC, Zhou YE, Lin TC. Potential antioxidant properties and hepatoprotective effects of an aqueous extract formula derived from three Chinese medicinal herbs against CCl4-induced liver injury in rats. Int Immunopharmacol. 2013 Jan;15(1):106-13. doi: 10.1016/j.intimp.2012.10.017. Epub 2012 Nov 8. PMID: 23142091.
Fiamegos YC, Kastritis PL, Exarchou V, Han H, Bonvin AM, Vervoort J, Lewis K, Hamblin MR, Tegos GP. Antimicrobial and efflux pump inhibitory activity of caffeoylquinic acids from Artemisia absinthium against gram-positive pathogenic bacteria. PLoS One. 2011 Apr 4;6(4):e18127. doi: 10.1371/journal.pone.0018127. PMID: 21483731; PMCID: PMC3070693.
Ali H, Kabir N, Muhammad A, Shah MR, Musharraf SG, Iqbal N, Nadeem S. Hautriwaic acid as one of the hepatoprotective constituent of Dodonaea viscosa. Phytomedicine. 2014 Jan 15;21(2):131-40. doi: 10.1016/j.phymed.2013.08.019. Epub 2013 Sep 24. PMID: 24075215.
Kanbur M, Eraslan G, Beyaz L, Silici S, Liman BC, Altinordulu S, Atasever A. The effects of royal jelly on liver damage induced by paracetamol in mice. Exp Toxicol Pathol. 2009 Mar;61(2):123-32. doi: 10.1016/j.etp.2008.06.003. Epub 2008 Aug 6. PMID: 18693095.
Wang X, Xu X, Tao W, Li Y, Wang Y, Yang L. A systems biology approach to uncovering pharmacological synergy in herbal medicines with applications to cardiovascular disease. Evid Based Complement Alternat Med. 2012;2012:519031. doi: 10.1155/2012/519031. Epub 2012 Nov 29. PMID: 23243453; PMCID: PMC3518963.
Rao ChV, Rawat AK, Singh AP, Singh A, Verma N. Hepatoprotective potential of ethanolic extract of Ziziphus oenoplia (L.) Mill roots against antitubercular drugs induced hepatotoxicity in experimental models. Asian Pac J Trop Med. 2012 Apr;5(4):283-8. doi: 10.1016/S1995-7645(12)60040-6. PMID: 22449519.
Tang X, Gao J, Wang Y, Fan YM, Xu LZ, Zhao XN, Xu Q, Qian ZM. Effective protection of Terminalia catappa L. leaves from damage induced by carbon tetrachloride in liver mitochondria. J Nutr Biochem. 2006 Mar;17(3):177-82. doi: 10.1016/j.jnutbio.2005.06.008. Epub 2005 Aug 10. PMID: 16169207.
Yue SM, Kang WY. Lowering blood lipid and hepatoprotective activity of amentoflavone from Selaginella tamariscina in vivo. J Med Plant Res. 2011 July 18;5(14):3007-3014.
Gong F, Yin ZH, Xu QT and Kang WY. Hepatoprotective effect of Mitragyna rotundifolia Kuntze on CCl4-induced acute liver injury in mice. Afr J Pharm Pharmacol. 2012;6(5):330-335. doi: 10.5897/AJPP11.766.
Newman DJ, Cragg GM, Snader KM. The influence of natural products upon drug discovery. Nat Prod Rep. 2000 Jun;17(3):215-34. doi: 10.1039/a902202c. PMID: 10888010.
Aghel N, Rashidi I, Mombeini A. Hepatoprotective Activity of Capparis spinosa root bark against CCl4 induced hepatic damage in mice. Iran J Pharm Res. 2007 Sempt;6(4):285-290.
Ritesh KR, Suganya A, Dileepkumar HV, Rajashekar Y, Shivanandappa T. A single acute hepatotoxic dose of CCl4 causes oxidative stress in the rat brain. Toxicol Rep. 2015 Jun 11;2:891-895. doi: 10.1016/j.toxrep.2015.05.012. PMID: 28962426; PMCID: PMC5598138.
Kim EY, Kim EK, Lee HS, Sohn Y, Soh Y, Jung HS, Sohn NW. Protective effects of Cuscutae semen against dimethylnitrosamine-induced acute liver injury in Sprague-Dawley rats. Biol Pharm Bull. 2007 Aug;30(8):1427-31. doi: 10.1248/bpb.30.1427. PMID: 17666798.
Ghosh A, Ghosh T, Jain S. Silymarin: A review on the pharmacodynamics and bioavailability enhancement approaches. Journal of Pharmaceutical Science and Technology. 2010;2(10):348-355.
Ahsan R, Islam KM, Balbul IJ, Musaddik MA, Haque E. Hepatoprotective activity of methanol extract of some medicinal plants against carbon tetrachloride-induced hepatotoxicity in rats. European Journal of Scientific Research. 2009;37(2):302-310.
Krishna KI, Mruthunjaya K, Patel JA. Antioxidant and hepatoprotective potential of stem methanolic extracts of Juticia gendarussa Burn Int J Pharm. 2010;6(2):72-80. doi: 10.3923/ijp.2010.72.80.
Hurkadale PJ, Shelar PA, Palled SG, Mandavkar YD, Khedkar AS. Hepatoprotective activity of Amorphophallus paeoniifolius tubers against paracetamol induced liver damage in rats. Asian Pac J Trop Biomed. 2012;1:238-242.