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Hepatoprotective Effect of Eulophia gracilis on Benzene-Induced Liver Toxicity in Wistar Rats

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Article Type Original Article
Subject Biology Group
OCLC JBRES Record
Olaniyi Solomon Ola* and Oyeronke Adunni Odunola
Issue: Volume4-Issue4
Pages: 665-677
Received: 2023-04-02
Accepted: 2023-04-06
Published: 2023-04-10

Abstract

Benzene (BZ) is an organic solvent that can induce pathological disorders such as haematological disburbance and liver damage. Eulophia gracilis (EG) plant is a medicinal orchid explored traditionally to treat myriads of ailments including anemia and liver disease. The present study investigated the protective effect of Eulophia gracilis extract against Benzene-induced hepatotoxicity in haematologically disturbed rats. Male Wistar rats (110-120 g) were randomized into four groups (n = 6 / group): I (control), II BZ-treated: 175 mg/kgbw benzene every alternate days for two weeks; III Co-treated with 175 mg/kgbw benzene and E. gracilis 200 mg/kgbw each day for two weeks and IV administered 200 mg/kg of E. gracilis for two weeks. BZ caused a significant reduction in PCV, Hb, RBC and altered white blood cell count. Similarly, activities of hepatic biomarker enzymes such as alkaline phosphatase, aspartate aminotransferase, alanine aminotransferase, and γ-glutamyl transferase were significantly increased in the BZ-treated group relative to control. Furthermore, BZ caused a significant decrease in the activities of hepatic arylesterase, superoxide dismutase, catalase and glutathione-S-transferase and levels of Ascorbic Acid (AA) and Glutathione (GSH). A significant increase in hepatic malondialdehyde content, altered liver ultrastructure and modulation of p53 and BCl-2 protein in the liver also accompanied benzene intoxication. However, coadministration of E. gracilis ameliorated the BZ-induced changes in haematological parameter and liver biomarkers and improved hepatic antioxidant biomarkers and ultrastructure. Overall, the results suggest that Eulophia gracilis protected against BZ-induced hepatic oxidative damage and haematological disturbance in rats.

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© 2023 Ola OS, et al. Distributed under Creative Commons CC-BY 4.0 Creative CommonsAttribution

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Ola OS, Odunola OA. Hepatoprotective Effect of Eulophia gracilis on Benzene-Induced Liver Toxicity in Wistar Rats. J Biomed Res Environ Sci. 2023 Apr 10; 4(4): 665-677. doi: 10.37871/jbres1724, Article ID: JBRES1724, Available at: https://www. jelsciences.com/articles/jbres1724.pdf

Subject area(s)

Biochemistry

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