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Overexpression miR-642 Inhibits Cell Migration and Proliferation by Regulation of KRT19 and Clinical Prognosis Value in Gastric Cancer

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Open Access
Article Type Research Article
Subject Biology Group
OCLC JBRES Record
Lan Mi, Yu Wu, Xing Wei, Xiaohua He, Xuanping Wang, Shaoheng Li and Yongping Mu*
Issue: Volume4-Issue4
Pages: 579-589
Received: 2023-03-27
Accepted: 2023-04-02
Published: 2023-04-03

Abstract

Objective: To investigate the expression of miR-642 in gastric cancer and its mechanism in the proliferation and migration.

Methods: qRT-PCR was used to detect the expression of miR-642 in gastric cancer. Dual luciferase gene reporting system was used to verify the direct regulatory effect of miR-642 on KRT19 3' UTR. The mRNA and protein levels of KRT19 gene after transfection were detected by qRT-PCR and Western blot. The proliferation of miR-642 was detected by IncucyteS long term dynamic cell observation. Kaplan-Meier method was used to analyze the relationship between miR-642 expression level and prognosis in patients with gastric cancer.

Results: The expression of miR-642 in gastric cancer tissues was lower than that in paracancer tissues. The fluorescence intensity of MGC803 cells was significantly decreased after co-transfection of miR-642 mimics and wild-type KRT19-3 'UTR recombinant vector. The expression levels of KRT19 mRNA and protein were significantly decreased after transfected with miR-642 mimics and KRT19 siRNA. Western blot analysis of PTEN, Akt and mTOR proteins showed different expression. Prognostic survival analysis showed that gastric cancer patients with low expression of miR-642 had shorter survival.

Conclusion: miR-642 expression can negatively regulate the target gene KRT19.

FullText HTML FullText PDF DOI: 10.37871/jbres1713 Crossmark

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Copyright

© 2023 Mi L, et al. Distributed under Creative Commons CC-BY 4.0 Creative CommonsAttribution

How to cite this article

Mi L, Wu Y, Wei X, He X, Wang X, Li S, Mu Y. Overexpression miR-642 Inhibits Cell Migration and Proliferation by Regulation of KRT19 and Clinical Prognosis Value in Gastric Cancer. 2023 Apr 03; 4(4): 579-589. doi: 10.37871/jbres1713, Article ID: JBRES1713, Available at: https://www.jelsciences.com/articles/jbres1713.pdf

Subject area(s)

Molecular BiomarkersBiology

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