Studying free Proline and Soluble Sugars Accumulation in the Fungus Aspergillus creber Exposed to Salt Stress

John Pouris*; Konstantina Kapnopoulou; Sofia Papadopoulou; Ioanna Pyrri

**John Pouris*, Konstantina Kapnopoulou, Sofia Papadopoulou and Ioanna Pyrri**

Volume4-Issue3
Dates: Received: 2023-02-11 | Accepted: 2023-03-13 | Published: 2023-03-16
Pages: 440-445

Abstract

Salinity is one of the most important factors influencing plant growth by participating in abiotic stress. Plants have developed a number of physiological mechanisms responding to abiotic stress. A well-studied response is the concentration of free proline and soluble sugars, which through various mechanisms enhances plant resistance to abiotic stress. What is less studied is that the mechanism of proline and sugars is present in other categories of organisms such as fungi. There are even less bibliographic data on a relationship of the mechanism of proline and soluble sugars accumulation in fungi. The purpose of this study is to investigate proline and soluble sugars accumulation in the fungus Aspergillus creber under salt stress tolerance.

FullText HTML FullText PDF DOI: 10.37871/jbres1693

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How to cite this article

Pouris J, Kapnopoulou K, Papadopoulou S, Pyrri I. Studying free Proline and Soluble Sugars Accumulation in the Fungus Aspergillus creber Exposed to Salt Stress. 2023 Mar 16; 4(3): 440-445. doi: 10.37871/jbres1693, Article ID: JBRES1693, Available at: https://www.jelsciences.com/articles/jbres1693.pdf

Subject area(s)

Microbiology

References

Sklenář F, Glässnerová K, Jurjević Ž, Houbraken J, Samson RA, Visagie CM, Yilmaz N, Gené J, Cano J, Chen AJ, Nováková A, Yaguchi T, Kolařík M, Hubka V. Taxonomy of Aspergillus series Versicolores: species reduction and lessons learned about intraspecific variability. Stud Mycol. 2022 Dec;102:53-93. doi: 10.3114/sim.2022.102.02. Epub 2022 Nov 16. PMID: 36760461; PMCID: PMC9903908.
Jurjevic Z, Peterson SW, Horn BW. Aspergillus section Versicolores: nine new species and multilocus DNA sequence based phylogeny. IMA Fungus. 2012 Jun;3(1):59-79. doi: 10.5598/imafungus.2012.03.01.07. Epub 2012 Jun 21. PMID: 23155501; PMCID: PMC3399103.
Jurjević Z, Peterson SW, Solfrizzo M, Peraica M. Sterigmatocystin production by nine newly described Aspergillus species in section Versicolores grown on two different media. Mycotoxin Res. 2013 Aug;29(3):141-5. doi: 10.1007/s12550-013-0160-4. Epub 2013 Feb 17. PMID: 23417508.
Rhizopoulou S, Diamantoglou ST, Passiakou l. Free proline accumulation in leaves, stems and roots of four mediterranean native phrygana species. Acta Oecologica. 1990;11(4):585-593.
Maurizio T, Roberto M, Paolo C. Multiple roles of proline in plant stress tolerance and development. Rendiconti Lincei. 2008;19:325-346. doi: 10.1007/s12210-008-0022-8.
John P, Meletiou-Christou M, Chimona C, Rhizopoulou S. Seasonal functional partitioning of carbohydrates and proline among plant parts of the sand daffodil. Agronomy. 2020;10(4):539. doi: 10.3390/agronomy10040539.
Chysanthi C, Sophia R. Water economy through matching plant root elongation to Mediterranean landscapes. World Journal of Research and Review. 2017;5:22-24.
Rosa M, Prado C, Podazza G, Interdonato R, González JA, Hilal M, Prado FE. Soluble sugars-metabolism, sensing and abiotic stress: a complex network in the life of plants. Plant Signal Behav. 2009 May;4(5):388-93. doi: 10.4161/psb.4.5.8294. Epub 2009 May 26. PMID: 19816104; PMCID: PMC2676748.
Hare PD, Cress WA. Metabolic implications of stress-induced proline accumulation in plants. Plant Growth Regulation. 1997;21:79-102. doi: 10.1023/A:1005703923347.
Roberto M, Paolo C.
Maurizio T, Costantino P, Mattioli R. Proline accumulation in plants: not only stress. Plant Signaling & Behavior. 2009;4(11):1016-1018. doi: 10.4161/psb.4.11.9797.
Krasensky J, Jonak C. Drought, salt, and temperature stress-induced metabolic rearrangements and regulatory networks. J Exp Bot. 2012 Feb;63(4):1593-608. doi: 10.1093/jxb/err460. Epub 2012 Jan 30. PMID: 22291134; PMCID: PMC4359903.
Szabados L, Savouré A. Proline: a multifunctional amino acid. Trends Plant Sci. 2010 Feb;15(2):89-97. doi: 10.1016/j.tplants.2009.11.009. Epub 2009 Dec 23. PMID: 20036181.
Rhizopoulou S, Mitrakos K. Water relations of evergreen sclerophylls. I. Seasonal changes in the water relations of eleven species from the same environment. Annals of Botany. 1990;65(2):171-178. doi: 10.1093/oxfordjournals.aob.a087921.
Munns R. Comparative physiology of salt and water stress. Plant Cell Environ. 2002 Feb;25(2):239-250. doi: 10.1046/j.0016-8025.2001.00808.x. PMID: 11841667.
Ben Rejeb K, Abdelly C, Savouré A. La proline, un acide aminé multifonctionnel impliqué dans l'adaptation des plantes aux contraintes environnementales [Proline, a multifunctional amino-acid involved in plant adaptation to environmental constraints]. Biol Aujourdhui. 2012;206(4):291-9. French. doi: 10.1051/jbio/2012030. Epub 2013 Feb 19. PMID: 23419256.
Liang X, Zhang L, Natarajan SK, Becker DF. Proline mechanisms of stress survival. Antioxid Redox Signal. 2013 Sep 20;19(9):998-1011. doi: 10.1089/ars.2012.5074. Epub 2013 May 23. PMID: 23581681; PMCID: PMC3763223.
Miransari M. Contribution of arbuscular mycorrhizal symbiosis to plant growth under different types of soil stress. Plant Biol (Stuttg). 2010 Jul 1;12(4):563-9. doi: 10.1111/j.1438-8677.2009.00308.x. PMID: 20636898.
Nehls U, Göhringer F, Wittulsky S, Dietz S. Fungal carbohydrate support in the ectomycorrhizal symbiosis: a review. Plant Biol (Stuttg). 2010 Mar;12(2):292-301. doi: 10.1111/j.1438-8677.2009.00312.x. PMID: 20398236.
Willetts HJ. The survival of fungal sclerotia under adverse environmental conditions. Biological Reviews. 1971;46(3):387-407. doi: 10.1111/j.1469-185X.1971.tb01050.x.
Chen C, Dickman MB. Proline suppresses apoptosis in the fungal pathogen Colletotrichum trifolii. Proc Natl Acad Sci U S A. 2005 Mar 1;102(9):3459-64. doi: 10.1073/pnas.0407960102. Epub 2005 Feb 7. PMID: 15699356; PMCID: PMC552905.
Rodaki A, Bohovych IM, Enjalbert B, Young T, Odds FC, Gow NA, Brown AJ. Glucose promotes stress resistance in the fungal pathogen Candida albicans. Mol Biol Cell. 2009 Nov;20(22):4845-55. doi: 10.1091/mbc.e09-01-0002. Epub 2009 Sep 16. PMID: 19759180; PMCID: PMC2777113.
Alwhibi MS, HashemA, Abd_AllahEF, Alqarawi AA , Soliman DWK , Wirth S, Egamberdieva D. Increased resistance of drought by trichoderma harzianum fungal treatment correlates with increased secondary metabolites and proline content. Journal of integrative agriculture. 2017;16(8):1751-1757. doi: 10.1016/S2095-3119(17)61695-2.
Gournas C, Evangelidis T, Athanasopoulos A, Mikros E, Sophianopoulou V. The Aspergillus nidulans proline permease as a model for understanding the factors determining substrate binding and specificity of fungal amino acid transporters. J Biol Chem. 2015 Mar 6;290(10):6141-55. doi: 10.1074/jbc.M114.612069. Epub 2015 Jan 8. PMID: 25572393; PMCID: PMC4358254.
Fomicheva GM, Vasilenko OV, Marfenina OE. [Comparative morphological, ecological, and molecular studies of Aspergillus versicolor (Vuill.) Tiraboschi strains isolated from different ecotopes]. Mikrobiologiia. 2006 Mar-Apr;75(2):228-34. Russian. PMID: 16758871.
Polo J, Mata P. Evaluation of a Biostimulant (Pepton) Based in Enzymatic Hydrolyzed Animal Protein in Comparison to Seaweed Extracts on Root Development, Vegetative Growth, Flowering, and Yield of Gold Cherry Tomatoes Grown under Low Stress Ambient Field Conditions. Front Plant Sci. 2018 Jan 19;8:2261. doi: 10.3389/fpls.2017.02261. PMID: 29403513; PMCID: PMC5780448.
Bates LS, Waldren RP, Teare ID. Rapid determination of free proline for water-stress studies. Plant and Soil. 1973. doi: 10.1007/BF00018060.
DuBois M, Gilles KA, Hamilton JK, Rebers PT, Smith F. Colorimetric method for determination of sugars and related substances. Analytical chemistry. 1956;28(3):350-356. doi: 10.1021/ac60111a017.
Buysse J, Merckx R. An improved colorimetric method to quantify sugar content of plant tissue. Journal of Experimental Botany, 1993;44(10):1627-1629. doi: 10.1093/jxb/44.10.1627.
Li X, Han S, Wang G, Liu X, Amombo E, Xie Y, Fu J. The Fungus Aspergillus aculeatus Enhances Salt-Stress Tolerance, Metabolite Accumulation, and Improves Forage Quality in Perennial Ryegrass. Front Microbiol. 2017 Sep 4;8:1664. doi: 10.3389/fmicb.2017.01664. PMID: 28936200; PMCID: PMC5595160.