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Three-Dimensional Electron Microscopy of Human Umbilical Cord Tissue Allograft Pre and Post Processing: A Literature Comparison

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Open Access
Article Type Research Article
Subject Biology Group
OCLC JBRES Record
Justine M Davis*, Joseph R Purita, John Shou and Tyler C Barrett
Issue: Volume3-Issue8
Pages: 934-940
Received: 2022-08-05
Accepted: 2022-08-14
Published: 2022-08-15

Abstract

One in four adults in the US suffer from cartilage degeneration of the Intervertebral Disc (DDD) or load bearing joints (DJD). Since cartilage is avascular, it has a limited regenerative capacity. Conventional non-surgical treatments provide brief symptomatic relief, have sided effects, and do not address the cartilage defect itself. As such, new alternatives are needed. Perinatal birth tissue allografts are a novel frontier for bio-mechanical cartilage engineering research. The tissues of interest include umbilical cord-derived Wharton’s Jelly (WJ). This study assessed WJ tissue samples via ZEISS Supra 55VP FieldEmission Scanning Electron Microscope (SEM) at 100 and 300 nm resolution scales. The captured images of pre and post-processed structural tissue matrices in WJ allografts were analyzed against themselves and peer-reviewed SEM images of articular cartilage, intervertebral disc cartilage, and muscle fascia. SEM images of post-processed WJ structural tissue matrices were found to be comparable to structural tissue matrices in human articular cartilage, intervertebral disc cartilage, and muscle fascia on a qualitative and quantitative level. This is the first study that we are aware of, to demonstrate that structural collagen matrices in post-processed WJ allografts are analogous in structure to the cartilage in articular joints, intervertebral discs, and muscle fascia.

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Copyright

© 2022 Davis JM, et al. Distributed under Creative Commons CC-BY 4.0 Creative CommonsAttribution

How to cite this article

Davis JM, Purita JR, Shou J, Barrett TC. Three-Dimensional Electron Microscopy of Human Umbilical Cord Tissue Allograft Pre and Post Processing: A Literature Comparison. J Biomed Res Environ Sci. 2022 Aug 15; 3(8): 934-940. doi: 10.37871/jbres1535, Article ID: JBRES1535, Available at: https://www.jelsciences.com/articles/jbres1535.pdf

Subject area(s)

Biochemistry

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