Yao-Feng Hu, Hao-Yu Liu* and Te-Lung Tsai
Volume3-Issue5
Dates: Received: 2022-05-05 | Accepted: 2022-05-29 | Published: 2022-05-29
Pages: 659-663
Abstract
The complete laboratory data of 24 samples with confirmed coronavirus Disease-2019 were retrieved by nucleic acid testing, and the quantification of SARS-CoV-2 with the World Health Organization (WHO) SARS-CoV-2 envelope (E-gene), RNA-Dependent RNA Polymerase (RdRP-gene), Nucleocapsid (N-gene) three primers by the Roche Cobas z 480. Throat or nasopharyngeal swabs reports demonstrated three primer Cross Points (CPs) of greater than 29, but less than 29 in sputum. COVID-19 confirmed patients demonstrated three genes E-, RdRp- and N-genes, actually four of 24 no showed N-gene, highly suspected to be weakly positive. яндекс
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DOI: 10.37871/jbres1492
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© 2022 Yao-Feng H, et al. Distributed under Creative Commons CC-BY 4.0 
How to cite this article
Yao-Feng H, Hao-Yu L, Te-Lung T. Four N-Genes in Using RT-PCR to Confirmed Coronavirus Disease-2019 (COVID-19) Pneumonia. J Biomed Res Environ Sci. 2022 May 29; 3(5): 659-663. doi: 10.37871/jbres1492, Article ID: JBRES1492, Available at: https://www.jelsciences.com/articles/jbres1492.pdf
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References
- Ye ZW, Yuan S, Yuen KS, Fung SY, Chan CP, Jin DY. Zoonotic origins of human coronaviruses. Int J Biol Sci. 2020 Mar 15;16(10):1686-1697. doi: 10.7150/ijbs.45472. PMID: 32226286; PMCID: PMC7098031.
- Carter JG, Orueta Iturbe L, Duprey JHA, Carter IR, Southern CD, Rana M, Whalley CM, Bosworth A, Beggs AD, Hicks MR, Tucker JHR, Dafforn TR. Ultrarapid detection of SARS-CoV-2 RNA using a reverse transcription-free exponential amplification reaction, RTF-EXPAR. Proc Natl Acad Sci U S A. 2021 Aug 31;118(35):e2100347118. doi: 10.1073/pnas.2100347118. PMID: 34400545; PMCID: PMC8536344.
- Visseaux B, Le Hingrat Q, Collin G, Bouzid D, Lebourgeois S, Le Pluart D, Deconinck L, Lescure FX, Lucet JC, Bouadma L, Timsit JF, Descamps D, Yazdanpanah Y, Casalino E, Houhou-Fidouh N; Emergency Department Influenza Study Group. Evaluation of the QIAstat-Dx Respiratory SARS-CoV-2 Panel, the First Rapid Multiplex PCR Commercial Assay for SARS-CoV-2 Detection. J Clin Microbiol. 2020 Jul 23;58(8):e00630-20. doi: 10.1128/JCM.00630-20. PMID: 32341142; PMCID: PMC7383528.
- Mardian Y, Kosasih H, Karyana M, Neal A, Lau CY. Review of Current COVID-19 Diagnostics and Opportunities for Further Development. Front Med (Lausanne). 2021 May 7;8:615099. doi: 10.3389/fmed.2021.615099. PMID: 34026773; PMCID: PMC8138031.
- Poljak M, Korva M, Knap Gašper N, Fujs Komloš K, Sagadin M, Uršič T, Avšič Županc T, Petrovec M. Clinical Evaluation of the cobas SARS-CoV-2 Test and a Diagnostic Platform Switch during 48 Hours in the Midst of the COVID-19 Pandemic. J Clin Microbiol. 2020 May 26;58(6):e00599-20. doi: 10.1128/JCM.00599-20. PMID: 32277022; PMCID: PMC7269406.
- Recommendations for COVID-19: Case definition, specimen collection, and Diagnostic Tests. April, 2020.
- Tang YW, Schmitz JE, Persing DH, Stratton CW. Laboratory Diagnosis of COVID-19: Current Issues and Challenges. J Clin Microbiol. 2020 May 26;58(6):e00512-20. doi: 10.1128/JCM.00512-20. PMID: 32245835; PMCID: PMC7269383.
- Chu DKW, Pan Y, Cheng SMS, Hui KPY, Krishnan P, Liu Y, Ng DYM, Wan CKC, Yang P, Wang Q, Peiris M, Poon LLM. Molecular Diagnosis of a Novel Coronavirus (2019-nCoV) Causing an Outbreak of Pneumonia. Clin Chem. 2020 Apr 1;66(4):549-555. doi: 10.1093/clinchem/hvaa029. PMID: 32031583; PMCID: PMC7108203.
- Wang X, Tan L, Wang X, Liu W, Lu Y, Cheng L, Sun Z. Comparison of nasopharyngeal and oropharyngeal swabs for SARS-CoV-2 detection in 353 patients received tests with both specimens simultaneously. Int J Infect Dis. 2020 May;94:107-109. doi: 10.1016/j.ijid.2020.04.023. Epub 2020 Apr 18. PMID: 32315809; PMCID: PMC7166099.
- Li Y, Yao L, Li J, Chen L, Song Y, Cai Z, Yang C. Stability issues of RT-PCR testing of SARS-CoV-2 for hospitalized patients clinically diagnosed with COVID-19. J Med Virol. 2020 Jul;92(7):903-908. doi: 10.1002/jmv.25786. Epub 2020 Apr 5. PMID: 32219885; PMCID: PMC7228231. яндекс
