Mohammad Jalil Zorriehzahra, Mina Ziarati* and Fatemeh Hassantabar
Volume3-Issue1
Dates: Received: 2022-01-03 | Accepted: 2022-01-06 | Published: 2022-01-11
Pages: 028-030
Abstract
The consumption of fish as useful animal protein is on the rise around the world, and the transport of fish is being considered as a means of trade between countries around the world. Every year, the occurrence of the Viral Necrosis Virus (VNN) disease in fish, as their main host, causes great damage to the fisheries industry. Undoubtedly, one of the ways to succeed in the aquaculture industry of the future will be the rapid diagnosis and prevention of aquatic diseases, which are currently the biggest cause of losses in the aquaculture industry. Failure to definitively diagnose the pathogen in aquatic animals has led to an increase in the use of antibiotics and, while it may not lead to definitive treatment, has also led to the development of drug resistance. Therefore, prolonging the time of identification and diagnosis of the pathogen causes more prevalence of the disease, more losses and damages in fish farms and the resulting costs. OIE documents and various pieces of evidence introduce valid tools and methods in the diagnosis of betanodavirus that can be used according to the type of sample and available facilities. In this regard, the selection of a valid and rapid diagnosis method will make a significant contribution to improving methods to control, prevent and combat the spread of this worldwide disease. Therefore, the purpose of this study is to compare different methods of VNN diagnosis and evaluate their advantages and disadvantages.
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DOI: 10.37871/jbres1397
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Copyright
© 2022 Zorriehzahra MJ, et al. Distributed under Creative Commons CC-BY 4.0 
How to cite this article
Zorriehzahra MJ, Ziarati M, Hassantabar F. Comparative Study of Different Methods for Viral Nervous Necrosis (VNN) Diagnosis in Fish. J Biomed Res Environ Sci. 2022 Jan 11; 3(1): 028-030. doi: 10.37871/jbres1397, Article ID: JBRES1397, Available at: https://www.jelsciences.com/articles/jbres1397.pdf
Subject area(s)
University/Institute
References
- Bellance R, Gallet D. L'encephalite virale loup mer Caraibes Med. 1988.
- OIE. Manual of Diagnostic Tests for Aquatic Animals. Chapter 2.3.12. Viral Encephalopathy and Retinopathy. 2017
- Banerjee D, Hamod MA, Suresh T, Karunasagar I. Isolation and characterization of a nodavirus associated with mass mortality in Asian seabass (Lates calcarifer) from the west coast of India. Virusdisease. 2014 Dec;25(4):425-9. doi: 10.1007/s13337-014-0226-8.Epub2014Aug31.PMID:25674617;PMCID:PMC4262316.
- Low CF, Syarul Nataqain B, Chee HY, Rozaini MZH, Najiah M. Betanodavirus: Dissection of the viral life cycle. J Fish Dis. 2017Nov;40(11):1489-1496.doi: 10.1111/jfd.12638.Epub2017Apr27.PMID:28449248.
- Nerland AH, Skaar C, Eriksen TB, Bleie H. Detection of nodavirus in seawater from rearing facilities for Atlantic halibut Hippoglossus hippoglossus larvae. Dis Aquat Organ. 2007 Jan 18;73(3):201-5. doi: 10.3354/dao073201. PMID: 17330739.
- Vendramin N, Patarnello P, Toffan A, Panzarin V, Cappellozza E, Tedesco P, Terlizzi A, Terregino C, Cattoli G. Viral Encephalopathy and Retinopathy in groupers (Epinephelus spp.) in southern Italy: a threat for wild endangered species? BMCVetRes. 2013Jan26;9:20.doi: 10.1186/1746-6148-9-20.PMID:23351980;PMCID:PMC3566913.
- Costa JZ, Thompson KD. Understanding the interaction between Betanodavirus and its host for the development of prophylactic measures for viral encephalopathy and retinopathy. Fish Shellfish Immunol. 2016 Jun;53:35-49. doi: 10.1016/j.fsi.2016.03.033.Epub2016Mar17.PMID:26997200.
- Zorriehzahra MJ. Viral nervous necrosis disease. In Emerging and reemerging viral pathogens. AcademicPress. 2020. doi: 10.1016/B978-0-12-819400-3.00030-2.
- Hassantabar, Fatemeh, Z M Jalil, Firoozbakhsh, Farid,Thompson, Kim, (2018). Feasibility study of constructing a rapid diagnosis kit for Viral Nervous Necrosis (VNN) for rapid detection of disease using immunochromatographic test and comparison with RT-PCR and immunohistochemistry (IHC), PhD thesis Aquaculture, Faculty of Animal Sciences and Fisheries, Sari University of Agricultural Sciences and Natural Resources. 2018.
- Panteghini M, Forest JC. Standardization in laboratory medicine: new challenges. Clin Chim Acta. 2005 May;355(1-2):1-12.doi: 10.1016/j.cccn.2004.12.003.PMID:15820472.
- Garibyan L and Avashia N. Research Techniques Made Simple: Polymerase Chain Reaction (PCR). J Invest Dermatol. 2013 March;133(3). doi: 10.1038/jid.2013.1.
- Favrot C. Polymerase chain reaction: Advantages and drawbacks. In: 3. Congresso Latinoamericano de Dermatologia Veterinaria, Buenos Aires, Argentina, 2015. doi: 10.5167/uzh-116536.
- Hajia M. Limitations of Different PCR Protocols Used in Diagnostic Laboratories: A Short Review. Modern Medical Laboratory Journal. ModMedLab. 2017;1(1):1-6. doi: 10.30699/mmlj17-01-01.
- Dalla VL, Zanella L, Patarnello P, Paolucci L, Belvedere P and Colombo L. Development of a sensitive diagnostic assay for fish nervous necrosis virus based on RT-PCR plus nested PCR. J Fish Dis. 2000;23:321-327. doi: 10.1046/j.1365-2761.2000.00255.x.
- Gomez DK, Sato J, Mushiake K, Isshiki T, Okinaka Y, Nakai T. PCR-based detection of betanodaviruses from cultured and wild marine fish with no clinical signs. J Fish Dis. 2004 Oct;27(10):603-8. doi: 10.1111/j.1365-2761.2004.00577.x. PMID: 15482425.
- Panzarin V, Patarnello P, Mori A, Rampazzo E, Cappellozza E, Bovo G, Cattoli G. Development and validation of a real-time TaqMan PCR assay for the detection of betanodavirus in clinical specimens. Arch Virol. 2010 Aug;155(8):1193-203.doi: 10.1007/s00705-010-0701-5.Epub2010Jun9.PMID:20532929.
- Lovatt A. Applications of quantitative PCR in the biosafety and genetic stability assessment of biotechnology products. J Biotechnol. 2002 Jan;82(3):279-300. doi: 10.1016/s1389-0352(01)00043-5. PMID: 11999695; PMCID: PMC7148957.
- Doan QK, Vandeputte M, Chatain B, Morin T, Allal F. Viral encephalopathy and retinopathy in aquaculture: a review. J Fish Dis. 2017 May;40(5):717-742. doi: 10.1111/jfd.12541. Epub 2016 Sep 16. PMID: 27633881.
