Covid-19 Research

Research Article

OCLC Number/Unique Identifier: 9227641184

Effects of Heavy Metal Toxicity on Anxiety Disorder

Medicine Group    Start Submission

Mfem CC, Seriki SA* and Oyama SE

Dates: Received: 2021-07-20 | Accepted: 2021-08-10 | Published: 2021-08-13
Pages: 660-668


Background: Several heavy metals are found naturally in the earth crust and are exploited for various industrial and economic purposes. Among these heavy metals, a few have impact on the human body. Though some of these metals only have effect on human physiology in high doses, others such as cadmium, mercury, lead, chromium, silver, and arsenic have delirious effects in the body even in minute quantities, causing acute and chronic toxicities in human. Anxiety is a common psychiatric disorder among men and women. Occasional anxiety may be a normal a part of life. However, people with anxiety disorders frequently have intense, excessive and persistent worry and fear about everyday situations. Often, anxiety disorders involve repeated episodes of sudden feelings of intense anxiety and fear or terror that reach a peak within minutes (panic attacks). This study examined a major environmental risk factor of anxiety disorder and how the adverse impact of anxiety disorder could be ameliorated using zinc and vitamin E.

Methods: Animals used for the researched were grouped into four. Group 1 represents control group; Group 2 represents animals exposed a heavy metal (CaCl2); Group 3 represents animals exposed to CaCl2 and then treated with zinc; and Group 4 represents animals exposed to CaCl2 and then treated vit E.

Results: Results showed that exposure to heavy metals (CaCl2 in particular) causes severe anxiety disorders. Results also showed zinc and vit E have the capacity to ameliorate anxiety disorder caused by heavy metals.

Conclusion: The human body should be adequately protected as man interacts with heavy metals to prevent anxiety disorder, and where a patient becomes a victim of the disorder, zinc and vit E could be used to manage the case.

FullText HTML FullText PDF DOI: 10.37871/jbres1294

Certificate of Publication


© 2021 Mfem CC, et al. Distributed under Creative Commons CC-BY 4.0

How to cite this article

Mfem CC, Seriki SA, Oyama SE. Effects of Heavy Metal Toxicity on Anxiety Disorder. J Biomed Res Environ Sci. 2021 Aug 13; 2(8): 660-668. doi: 10.37871/jbres1294, Article ID: JBRES1294, Available at:

Subject area(s)


  1. Fergusson, J E. The heavy elements: chemistry, environmental impact and health effects. Oxford: pergamon press.
  2. He ZL, Yang XE, Stoffella PJ. Trace elements in agroecosystems and impacts on the environment. J Trace Elem Med Biol. 2005;19(2-3):125-40. doi: 10.1016/j.jtemb.2005.02.010. Epub 2005 Oct 24. PMID: 16325528.
  3. Nriagu JO. A global assessment of natural sources of atmospheric trace metals. Nature. 1989;338:47-49.
  4. Bradl H. Heavy metals in the environment: Origin, interaction and remediation. London: Academic press. 2002.Vol 6.
  5. Wang S, Shi X. Molecular mechanisms of metal toxicity and carcinogenesis. Mol Cell Biochem. 2001 Jun;222(1-2):3-9. PMID: 11678608.
  6. Burger J. Assessment and management of risk to wildlife from cadmium. Sci Total Environ. 2008 Jan 15;389(1):37-45. doi: 10.1016/j.scitotenv.2007.08.037. Epub 2007 Oct 29. PMID: 17910979.
  7. Zadorozhnaja TD, Little RE, Miller RK, Mendel NA, Taylor RJ, Presley BJ, Gladen BC. Concentrations of arsenic, cadmium, copper, lead, mercury, and zinc in human placentas from two cities in Ukraine. J Toxicol Environ Health A. 2000 Oct 27;61(4):255-63. doi: 10.1080/00984100050136571. PMID: 11071319.
  8. Benoff S, Jacob A, Hurley IR. Male infertility and environmental exposure to lead and cadmium. Hum Reprod Update. 2000 Mar-Apr;6(2):107-21. doi: 10.1093/humupd/6.2.107. PMID: 10782569.
  9. WHO. Cadmium (Environmental Health Criteria No. 134). Geneva. 1992.
  10. Järup L, Akesson A. Current status of cadmium as an environmental health problem. Toxicol Appl Pharmacol. 2009 Aug 1;238(3):201-8. doi: 10.1016/j.taap.2009.04.020. Epub 2009 May 3. PMID: 19409405.
  11. Wilson DN. Association cadmium. Cadmium market trends and influences; London. Cadmium 87 proceedings of the 6th international cadmium conference. 1988;9-16.
  12. Takaki A, Jimi S, Segawa M, Hisano S, Takebayashi S, Iwasaki H. Long-term cadmium exposure accelerates age-related mitochondrial changes in renal epithelial cells. Toxicology. 2004 Oct 15;203(1-3):145-54. doi: 10.1016/j.tox.2004.06.005. PMID: 15363590.
  13. Seidal K, Jörgensen N, Elinder CG, Sjögren B, Vahter M. Fatal cadmium-induced pneumonitis. Scand J Work Environ Health. 1993 Dec;19(6):429-31. doi: 10.5271/sjweh.1450. PMID: 8153597.
  14. Godt J, Scheidig F, Grosse-Siestrup C, Esche V, Brandenburg P, Reich A, Groneberg DA. The toxicity of cadmium and resulting hazards for human health. J Occup Med Toxicol. 2006 Sep 10;1:22. doi: 10.1186/1745-6673-1-22. PMID: 16961932; PMCID: PMC1578573.
  15. Zadorozhnaja TD, Little RE, Miller RK, Mendel NA, Taylor RJ, Presley BJ, Gladen BC. Concentrations of arsenic, cadmium, copper, lead, mercury, and zinc in human placentas from two cities in Ukraine. J Toxicol Environ Health A. 2000 Oct 27;61(4):255-63. doi: 10.1080/00984100050136571. PMID: 11071319.
  16. Shukla A, Shukla GS, Srimal RC. Cadmium-induced alterations in blood-brain barrier permeability and its possible correlation with decreased microvessel antioxidant potential in rat. Hum Exp Toxicol. 1996 May;15(5):400-5. doi: 10.1177/096032719601500507. PMID: 8735464.
  17. Okuda B, Iwamoto Y, Tachibana H, Sugita M. Parkinsonism after acute cadmium poisoning. Clin Neurol Neurosurg. 1997 Dec;99(4):263-5. doi: 10.1016/s0303-8467(97)00090-5. PMID: 9491302.
  18. Cao Y, Chen A, Radcliffe J, Dietrich KN, Jones RL, Caldwell K, Rogan WJ. Postnatal cadmium exposure, neurodevelopment, and blood pressure in children at 2, 5, and 7 years of age. Environ Health Perspect. 2009 Oct;117(10):1580-6. doi: 10.1289/ehp.0900765. Epub 2009 May 26. PMID: 20019909; PMCID: PMC2790513.
  19. Brenneman KA, Wong BA, Buccellato MA, Costa ER, Gross EA, Dorman DC. Direct olfactory transport of inhaled manganese ((54)MnCl(2)) to the rat brain: toxicokinetic investigations in a unilateral nasal occlusion model. Toxicol Appl Pharmacol. 2000 Dec 15;169(3):238-48. doi: 10.1006/taap.2000.9073. PMID: 11133346.
  20. TSUI C. The role of zinc auxin synthesis in the tomato plant. Am J Bot. 1948 Mar;35(3):172-9. PMID: 18909962.
  21. Powell SR. Antioxidant properties of zinc. Journal of Nutrition. 2000;130:1447s-1454s. doi: 10.1093/jn/130.5.1447S
  22. Ozdemir G, Inanc F. Zinc may protect remote ocular injury caused by intestinal ischemia reperfusion in rats. Tohoku J Exp Med. 2005 Jul;206(3):247-51. doi: 10.1620/tjem.206.247. PMID: 15942153.
  23. Niki E, Traber MG. A history of vitamin E. Ann Nutr Metab. 2012;61(3):207-12. doi: 10.1159/000343106. Epub 2012 Nov 26. PMID: 23183290.
  24. Di Mascio P, Murphy ME, Sies H. Antioxidant defense systems: the role of carotenoids, tocopherols, and thiols. Am J Clin Nutr. 1991 Jan;53(1 Suppl):194S-200S. PMID: 1985387.
  25. Galli F, Azzi A, Birringer M, Cook-Mills JM, Eggersdorfer M, Frank J, Cruciani G, Lorkowski S, Özer NK. Vitamin E: Emerging aspects and new directions. Free Radic Biol Med. 2017 Jan;102:16-36. doi: 10.1016/j.freeradbiomed.2016.09.017. Epub 2016 Nov 2. PMID: 27816611.
  26. Rimbach G, Moehring J, Huebbe P, Lodge JK. Gene-regulatory activity of alpha-tocopherol. Molecules. 2010 Mar 12;15(3):1746-61. doi: 10.3390/molecules15031746. PMID: 20336011; PMCID: PMC6257188.
  27. Azzi A. Many tocopherols, one vitamin E. Mol Aspects Med. 2018 Jun;61:92-103. doi: 10.1016/j.mam.2017.06.004. Epub 2017 Jun 19. PMID: 28624327.
  28. American Psychiatric Association. Diagnostic and statistical manual of mental disorders. 5th ed. Arlington, VA: American psychiatric publishing. 2013. p. 189.
  29. Bouras N, Holt G. Psychiatric and behavioural disorders in intellectual and developmental disabilities. 2nd ed. Cambridge university press. 2007.
  30. Wells, Adrian. Metacognitive therapy for anxiety and depression. New York: Guilford press. 2011.
  31. Ojo JO, Mouzon B, Algamal M, Leary P, Lynch C, Abdullah L, Evans J, Mullan M, Bachmeier C, Stewart W, Crawford F. Chronic Repetitive Mild Traumatic Brain Injury Results in Reduced Cerebral Blood Flow, Axonal Injury, Gliosis, and Increased T-Tau and Tau Oligomers. J Neuropathol Exp Neurol. 2016 Jul;75(7):636-55. doi: 10.1093/jnen/nlw035. Epub 2016 May 31. PMID: 27251042; PMCID: PMC4913432.
  32. Carobrez AP, Bertoglio LJ. Ethological and temporal analyses of anxiety-like behavior: the elevated plus-maze model 20 years on. Neurosci Biobehav Rev. 2005;29(8):1193-205. doi: 10.1016/j.neubiorev.2005.04.017. Epub 2005 Aug 9. PMID: 16084592.
  33. Hogg S. A review of the validity and variability of the elevated plus-maze as an animal model of anxiety. Pharmacol Biochem Behav. 1996 May;54(1):21-30. doi: 10.1016/0091-3057(95)02126-4. PMID: 8728535.
  34. Tapiero H, Tew KD. Trace elements in human physiology and pathology: zinc and metallothioneins. Biomed Pharmacother. 2003 Nov;57(9):399-411. doi: 10.1016/s0753-3322(03)00081-7. PMID: 14652165.
  35. Brown RE, Corey SC, Moore AK. Differences in measures of exploration and fear in MHC - Congenic C57BL/6J and B6-H-2k mice. Behavioural Genetics. 1999;29:263-271. doi: 10.1023/A:1021694307672
  36. Ennaceur A. Tests of unconditioned anxiety - pitfalls and disappointments. Physiol Behav. 2014 Aug;135:55-71. doi: 10.1016/j.physbeh.2014.05.032. Epub 2014 Jun 5. PMID: 24910138.
  37. Bourin M, Hascoët M. The mouse light/dark box test. Eur J Pharmacol. 2003 Feb 28;463(1-3):55-65. doi: 10.1016/s0014-2999(03)01274-3. PMID: 12600702.
  38. Hascoët M, Bourin M, Nic Dhonnchadha BA. The mouse light-dark paradigm: a review. Prog Neuropsychopharmacol Biol Psychiatry. 2001 Jan;25(1):141-66. doi: 10.1016/s0278-5846(00)00151-2. PMID: 11263750.
  39. Berglund M, Akesson A, Nermell B, Vahter M. Intestinal absorption of dietary cadmium in women depends on body iron stores and fiber intake. Environ Health Perspect. 1994 Dec;102(12):1058-66. doi: 10.1289/ehp.941021058. PMID: 7713018; PMCID: PMC1567470.
  40. Hall CS. Emotional behaviour in the rat; defecation and urination as measures of individual differences in emotionality. Journal of comparative psychology. 1934;18:382-403. doi: 10.1037/h0071444


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