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Assessment of Nanosilver Hemocompatibility in Prehypertensive Salt-Induced Animal Model

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Open Access
Article Type Original Article
Subject Medicine Group
OCLC 9152969214
Ogechukwu K Uche*, Esiri F Ohiambe and Fabian C Amechina
Issue: Volume2-Issue7
Pages: 567-573
Received: 2021-06-26
Accepted: 2021-07-20
Published: 2021-07-21

Abstract

Aim: There are Conflicting reports on safety profile of nanoparticles on biological cells. This study evaluated the impact of nanosilver on hemocompatibility on salt-loaded rats.

Materials and Methods: Sprague-Dawley rats [(inbred) (120-140 g)] randomly divided into of 4 groups, (n = 6) were studied. Group 1(control) received normal rat chow and tap water, Group 2 received rat chow containing 8% NaCl [(salt-loaded rats (SLRs)]. Group 3 received rat chow + Nanosilver Solution (NS) 0.18 mL 10 ppm/kg/day. Group 4 comprised SLRs + NS. After 6 weeks oral gavage treatments, measurements of Blood pressure (Bp) and Heart Rate (HR) were by pressure transducer via cannulation of left common carotid artery following anaesthesia with urethane. HR was computed by the number of arterial pulse per 60 seconds. 5 ml of blood for WBC, PLATELETS, RBC, PCV, HB, MCH, MCHC and MCV analyses using automated haematology analyser and Osmotic fragility reactivity with standard spectrophotometer at 540 nm wavelength.

Results: Exposure of nanosilver to normotensive rats resulted in significantly lower RBC level compared with control, whereas RBC level in Salt-Loaded Co-Treated Nanosilver (SCNS) was comparable with the SLRs. The tenet was the same for HB, PCV, MCH and MCHC. Nanosilver induced leukopenia in normotensive compared with control and prevented WBC elevation in SCNS. Platelets significantly increased in Nanosilver-Treated Normotensive Rats (NTNRs) compared with control and decreased in SCNS. Osmotic burst resistance increased in NTNRs and decreased in cells from treated groups.

Conclusion: Chronic exposure of nanosilver to salt loaded rats alters haematological parameters which may worsen circulatory function and activate risk factors of cardiovascular disorders.

FullText HTML FullText PDF DOI: 10.37871/jbres1278 Crossmark

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Copyright

© 2021 Uche OK, et al. Distributed under Creative Commons CC-BY 4.0 Creative CommonsAttribution

How to cite this article

Uche OK, Ohiambe EF, Amechina FC. Assessment of Nanosilver Hemocompatibility in Prehypertensive Salt-Induced Animal Model. J Biomed Res Environ Sci. 2021 July 21; 2(7): 567-273. doi: 10.37871/jbres1278, Article ID: JBRES1278, Available at: https://www.jelsciences.com/articles/jbres1278.pdf

Subject area(s)

HypertensionBlood DisordersPhysiologyCardiovascular Diseases

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