Cytogenetic Study on Mitotic Cell Division in Allium cepa by Lead (Pb) and Chromium (Cr) Containing Bacterial Strain Isolated from Tannery Effluents of Bangladesh

Afrin Priya Talukder; Sarwat Tazrian; Md. Nazmul Haque; Shahriar Zaman; Md. Akhtar-E-Ekram*

Afrin Priya Talukder, Sarwat Tazrian, Md. Nazmul Haque, Shahriar Zaman and Md. Akhtar-E-Ekram*

Volume2-Issue2
Dates: Received: 2021-02-16 | Accepted: 2021-02-25 | Published: 2021-02-26
Pages: 084-090

Abstract

In the present study, a bacterial strain capable of Pb and Cr detoxification was isolated from tannery effluents which was identified as Myroides sp. through 16S rRNA gene sequencing. In the cytogenetic experiment, 100 and 600 µg/ml of lead and chromium were used as treatment for the root tips of Allium cepa and caused many chromosomal abnormalities such as abnormal chromosome position, damaged nucleus, breaks of chromosome bridges and fragments also occurred. Notably, sticky metaphase was found where sticky chromosomes indicated highly toxic, usually irreversible consequences leading to cell death. However, Myroides sp. treated supernatant, collected after day 7, used to treat Allium cepa tips showed less mitotic aberrations, nuclear degeneration and observed normal anaphase and telophase stage indicating possible metal detoxifying ability of the isolated strain. Furthermore, LC50 value was 64.63 μl/ml for Myroides sp.

FullText HTML FullText PDF DOI: 10.37871/jbres1193

Certificate of Publication

Copyright

How to cite this article

Talukder AP, Tazrian S, Md. Nazmul H, Zaman S, Md. Akhtar-E-Ekram. Cytogenetic Study on Mitotic Cell Division in Allium cepa by Lead (Pb) and Chromium (Cr) Containing Bacterial Strain Isolated from Tannery Effluents of Bangladesh. J Biomed Res Environ Sci. 2021 Feb 26; 2(2): 084-090. doi: 10.37871/jbres1193, Article ID: jbres1193

Subject area(s)

Microbiology

Biology

References

Chandra S, Chauhan LK, Murthy RC, Saxena PN, Pande PN, Gupta SK. Comparative biomonitoring of leachates from hazardous solid waste of two industries using Allium test. Sci Total Environ. 2005 Jul 15;347(1-3):46-52. doi: 10.1016/j.scitotenv.2005.01.002. PMID: 16084966.
Arambašić MB, Bjelić S, Subakov G. Acute toxicity of heavy metals (copper, lead, zinc), phenol and sodium on Allium cepa L., Lepidium sativum L. and Daphnia magna St.: Comparative investigations and the practical applications. Wat Res. 1995 Feb; 29(2): 497-503. doi:10.1016/0043-1354(94)00178-A.
Zhuang P, McBride MB, Xia H, Li N, Li Z. Health risk from heavy metals via consumption of food crops in the vicinity of Dabaoshan mine, South China. Sci Total Environ. 2009 Feb 15;407(5):1551-61. doi: 10.1016/j.scitotenv.2008.10.061. Epub 2008 Dec 9. PMID: 19068266.
Rodriguez-Cea A, Ayllon F, Garcia-Vazquez E. Micronucleus test in freshwater fish species: an evaluation of its sensitivity for application in field surveys. Ecotoxicol Environ Saf. 2003 Nov;56(3):442-8. doi: 10.1016/s0147-6513(03)00073-3. PMID: 14575685.
Singh V, Chauhan PK, Kanta R, Dhewa T, Kumar V. Isolation and characterization of Pseudomonas resistant to heavy metals contaminants. Int J Pharm Sci Rev Res. 2014;3(2):165-168.
Roy A, Queirolo E, Peregalli F, Mañay N, Martínez G, Kordas K. Association of blood lead levels with urinary F₂-8α isoprostane and 8-hydroxy-2-deoxy-guanosine concentrations in first-grade Uruguayan children. Environ Res. 2015 Jul;140:127-35. doi: 10.1016/j.envres.2015.03.001. Epub 2015 Apr 4. PMID: 25863186; PMCID: PMC4492803.
Shobana SN. Characterization of chromium bioremediation by Stenotrophomonas maltophilia SRS 05 isolated from tannery effluent. Int Res J Eng Technol. 2017 Feb;4(2):403-429.
Jacobs JA, Testa SM. Overview of chromium(VI) in the environment: background and history. In: Guertin J, Jacobs JA and Avakian CP (eds.). Chromium (VI) Handbook, Boca Raton; Fl CRC Press:2005, pp. 1-22.
Panda BB, Panda KK. Genotoxicity and mutagenicity of metals in plants. Physiol biochem of metal toxicity and tolerance in plant. 2002, pp. 395-414.
Bruins MR, Kapil S, Oehme FW. Microbial resistance to metals in the environment. Ecotoxicol Environ Saf. 2000 Mar;45(3):198-207. doi: 10.1006/eesa.1999.1860. PMID: 10702338s.
Shah SU. Importance of genotoxicity & S2A guidelines for genotoxicity testing for pharmaceuticals. IOSR J Pharm Bio Sci. 2012;1(2):43-54.
Solis PN, Wright CW, Anderson MM, Gupta MP, Phillipson JD. A microwell cytotoxicity assay using Artemia salina (brine shrimp). Planta Med. 1993 Jun;59(3):250-2. doi: 10.1055/s-2006-959661. PMID: 8316592.
Wierzbicka M. Lead in the apoplast of Allium cepa L. root tips-ultrastructural studies. Plant Sci. 1998 Apr 1; 133(1):105-119. doi: 10.1016/S0168-9452(98)00023-5.
Einicker-Lamas M, Mezian GA, Fernandes TB, Silva FL, Guerra F, Miranda K, Attias M, Oliveira MM. Euglena gracilis as a model for the study of Cu2+ and Zn2+ toxicity and accumulation in eukaryotic cells. Environ Pollut. 2002;120(3):779-86. doi: 10.1016/s0269-7491(02)00170-7. PMID: 12442801.
Pan J, Zhu M, Chen H. Aluminum-induced cell death in root-tip cells of barley. Environ Exp Bot. 2001 Aug;46(1):71-79. doi: 10.1016/s0098-8472(01)00083-1. PMID: 11378174.
Siddiquee S, Rovina K, Azad SA, Naher L, Suryani S, Chaikaew P. Heavy metal contaminants removal from wastewater using the potential filamentous fungi biomass: A review. J Microbial Bioch Technol. 2015;7(6):384-395. doi:10.4172/1948-5948.1000243.
Okolo NV, Olowolafe EA, Akawu I, Okoduwa SIR. Effects of industrial effluents on soil resources in Challawa industrial area, Kano, Nigeria. J Glob Ecol Environ. 2016 July;5(1):1-10.
Igiri BE, Okoduwa SIR, Idoko GO, Akabuogu EP, Adeyi AO, Ejiogu IK. Toxicity and Bioremediation of Heavy Metals Contaminated Ecosystem from Tannery Wastewater: A Review. J Toxicol. 2018 Sep 27;2018:2568038. doi: 10.1155/2018/2568038. PMID: 30363677; PMCID: PMC6180975.
Magesh N, Chandrasekar N, Soundranayagam JP. Delineation of groundwater potential zones in Theni district, Tamil Nadu, using remote sensing, GIS and MIF techniques. Geosci Frontiers. 2012 March;3(2):189-196. doi:10.1016/j.gsf.2011.10.007.
Le TT, Son MH, Nam IH, Yoon H, Kang YG, Chang YS. Transformation of hexabromocyclododecane in contaminated soil in association with microbial diversity. J Hazard Mater. 2017 Mar 5;325:82-89. doi: 10.1016/j.jhazmat.2016.11.058. Epub 2016 Nov 21. PMID: 27915102.
Holmes B, Snell JJ, Lapage SP. Flavobacterium odoratum: a species resistant to a wide range of antimicrobial agents. J Clin Pathol. 1979 Jan;32(1):73-7. doi: 10.1136/jcp.32.1.73. PMID: 429582; PMCID: PMC1145571.
El-Helow ER, Sabry SA, Amer RM. Cadmium biosorption by a cadmium resistant strain of Bacillus thuringiensis: regulation and optimization of cell surface affinity for metal cations. Biometals. 2000 Dec;13(4):273-80. doi: 10.1023/a:1009291931258. PMID: 11247032.
Clarridge JE 3rd. Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases. Clin Microbiol Rev. 2004 Oct;17(4):840-62, table of contents. doi: 10.1128/CMR.17.4.840-862.2004. PMID: 15489351; PMCID: PMC523561.
Sabat AJ, van Zanten E, Akkerboom V, Wisselink G, van Slochteren K, de Boer RF, Hendrix R, Friedrich AW, Rossen JWA, Kooistra-Smid AMDM. Targeted next-generation sequencing of the 16S-23S rRNA region for culture-independent bacterial identification - increased discrimination of closely related species. Sci Rep. 2017 Jun 13; 7(1):3434. doi: 10.1038/s41598-017-03458-6. PMID: 28611406; PMCID: PMC5469791.
Duesenberg RH, Bighorn E, Chlebowicz MA, Couto N, Ferdous M, García-Cobos S, Kooistra-Smid AM, Raangs EC, Rosema S, Veloo AC, Zhou K, Friedrich AW, Rossen JW. Application of next generation sequencing in clinical microbiology and infection prevention. J Biotechnol. 2017 Feb 10;243:16-24. doi: 10.1016/j.jbiotec.2016.12.022. Epub 2016 Dec 29. PMID: 28042011.
Jo JH, Kennedy EA, Kong HH. Research Techniques Made Simple: Bacterial 16S Ribosomal RNA Gene Sequencing in Cutaneous Research. J Invest Dermatol. 2016 Mar;136(3):e23-e27. doi: 10.1016/j.jid.2016.01.005. PMID: 26902128; PMCID: PMC5482524.
Ventura-Camargo BDC, Maltempi P, Marin-Morales M. The use of the cytogenetic to identify mechanisms of action of an azo dye in Allium cepa meristematic cells. J Environ Analytic Toxicol. 2011; 1(3): 1-12.
Bharali MK, Aran K, Arangham G, Chetry LB. Cytogenetic effect of lead acetate exposure on root tip cells of Allium cepa L. J Glob Biosci. 2013; 2(6): 217-221.
Matsumoto ST, Marin-Morales MA. Mutagenic potential evaluation of the water of a river that receives tannery effluent using the Allium cepa test system. Cytologia. 2004; 69(4): 399-408.
Seth CS, Chaturvedi PK, Misra V. Toxic effect of arsenate and cadmium alone and in combination on giant duckweed (Spirodela polyrrhiza L.) in response to its accumulation. Environ Toxicol. 2007 Dec;22(6):539-49. doi: 10.1002/tox.20292. PMID: 18000854.
Abbas AA, Mohamed SM, Zahir HA. Biosorption of heavy metals by Pseudomonas bacteria. Int Res J Eng Technol. 2016 Aug;3(8):1446-1450.
Mazid M, Datta BK, Nahar L, Sarker S. Assessment of antibacterial activity and brine shrimp toxicity of two Polygonum species. Ars Pharmceutica. 2008 Aug;49(2):127-134.
Green ER, Mecsas J. Bacterial Secretion Systems: An Overview. Microbiol Spectr. 2016 Feb;4(1):10.1128/microbiolspec.VMBF-0012-2015. doi: 10.1128/microbiolspec.VMBF-0012-2015. PMID: 26999395; PMCID: PMC4804464.