Development of a New Anti-Rabies Antiviral: Effective Treatment with Beclin-1 and Lifespan Prolongation

Homeira Parizad; Asghar Abdoli; Ehsan Mostafavi; Farzaneh Sheikholeslami*

Homeira Parizad, Asghar Abdoli, Ehsan Mostafavi and Farzaneh Sheikholeslami*

Volume4-Issue6
Dates: Received: 2023-05-21 | Accepted: 2023-06-13 | Published: 2023-06-16
Pages: 1057-1068

Abstract

Autophagy is a cellular mechanism that exerts antiviral effects in some virus-infected cells, while it can have opposite effects on some other cells. Rabies is an infectious disease caused by negative-stranded RNA virus. After the street Rabies Virus (RABV), the vector containing Beclin-1 were injected into the brains of mice. They were sacrificed to collect the samples. Next, the focus forming assay, TUNEL assay and q PCR were performed. The expression of MAP1LC3B, ATG5, and BECN1 genes and the relative mRNA expression of autophagy-related genes increased after in vivo transfection of the pIRES2-EGFP-Beclin-1 vector in test groups (p < 0.01). The number of viruses decreased in the brains of test group compared to the controls. Survival significantly increased in mice treated with the Beclin-1 containing vector compared to controls. The TUNEL assay did not indicate apoptosis. LC3 was notably expressed in the brain samples that were previously transfected with the pIRES-EGFP-Beclin-1 vector.

FullText HTML FullText PDF DOI: 10.37871/jbres1768

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Parizad H, Abdoli A, Mostafavi E, Sheikholeslami F. Development of a New Anti-Rabies Antiviral: Effective Treatment with Beclin-1 and Lifespan Prolongation. 2023 June 16; 4(6): 1057-1068. doi: 10.37871/jbres1768, Article ID: JBRES1768, Available at: https://www.jelsciences.com/articles/jbres1768.pdf

Subject area(s)

Antivirology

Virology

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