Third-degree burn wounds can cause devastating loss of functionality for patients. As such, platform technologies that allow effective wound management are needed. Herein, we describe the use of MatriStem™ Urinary Bladder Matrix (UBM)-based products (MicroMatrix® + Cytal®) and a collagen and glycosaminoglycan (GAG) product (Integra® Dermal Regeneration Template (DRT)) in a porcine third-degree burn wound model. Full-thickness third-degree burns were created on dorsal porcine skin, debrided using an excisional model, then treated with MicroMatrix® + Cytal® or Integra® DRT; treatment groups were compared against the untreated wound controls. MicroMatrix® + Cytal®-treated wounds appeared to have faster wound closure and epithelialization compared to untreated and Integra® DRT-treated wounds. Multi-factor wound healing analysis demonstrated that MicroMatrix® + Cytal® performed better than untreated and Integra® DRT-treated wounds based on the metrics of contraction, epithelialization, and wound closure rate. Histological analysis demonstrated that all treated wounds had complete epidermal closure at various levels of maturation and presented mature, thick, and organized collagen bundles. Integra® DRT -treated wounds appeared to have more similar histological resemblance to native tissue compared to MicroMatrix® + Cytal® and untreated control. Importantly, treatment with either MicroMatrix® + Cytal® products or Integra® DRT, resulted in an extracellular matrix (ECM) composition and cellular organization similar to healthy skin. MicroMatrix®+ Cytal®-treatment promoted rapid wound closure and neo-epithelialization, while Integra® DRT-treatment seemed superior in promoting a healthy and pro-regenerative dermal wound bed.