Nanosilver (AgNPs) is widely used in various manufacturing sectors, including agriculture, food processing, and medicine. Many consumer products used in everyday life release silver either as ions or nanoparticles. Without proper regulation, consumption of Ag-containing materials will lead to significant risks for ecosystems and humans. Nonspecific oxidative stress is considered one of the most serious problems associated with nanoparticle toxicity. In this regard, research into the use of antioxidant drugs to prevent and protect against the negative consequences caused by exposure to Ag NPs on the body is promising. The purpose of this work was to evaluate the effect of the antioxidant drug Mexidol on the motor behaviour of rats that received daily Ag NPS with drinking water. For two weeks, rats in the 1 experimental group received an aqueous solution with 10-12 nm particles added to their drinking water. The daily dose of Ag NPs was 15.75 mg/kg. The second group of rats additionally received Mexidol, an antioxidant drug, intraperitoneally at a dose of 10 mg/kg as a 0.5% solution twice daily. All animals were tested in an Open Field (OF) on the first and 15th day of the experiment. During the final testing in the OF, urine samples were collected from the rats using dry chemistry test strips. Rats receiving Ag NPs in their drinking water for two weeks urine analysis revealed significant impairment of renal function. Chronic exposure to Ag NPs can negatively impact fundamental behavioural patterns in rats, particularly their stress responses and locomotor activity. In 1 group of rats defined a decrease in locomotor activity and an increase in anxiety-like behaviour compared to the first day of the experiment. In rats additionally receiving Mexidol injections, the drug improved exploratory behaviour and reduced emotional responses