Honeys popularity as a treatment is growing by the day, with many societies emphasizing its importance and its role in infections causing burns and wounds and recently gets focus from the medical community; as antimicrobial resistance is a rising issue. This study targets to check the antimicrobial activity of both red and white honey against two bacterial species (Staphylococcus aureus, Clostridium perfringens) and two fungal species (Trichophyton rubrum, Pencillium chrysogenum). Four honey dilutions for each type of honey were prepared and tested for its antimicrobial activity using agar well diffusion method. Both red honey and white honey showed effective antibacterial activity against C. perfringens with range of inhibition zone (16-22), (8-12) and antifungal activity against T. rubrum (24-30), (26.5-29) and P. chrysogenum (20-23), (18-21) respectively. While both honey types do not show any activity against S. aureus. So honey can serve as a wide range antimicrobial alternative against pathogens including medically important ones like clostridium and several superficial disease causing fungi like dermatophytes.