Salinity is one of the most important factors influencing plant growth by participating in abiotic stress. Plants have developed a number of physiological mechanisms responding to abiotic stress. A well-studied response is the concentration of free proline and soluble sugars, which through various mechanisms enhances plant resistance to abiotic stress. What is less studied is that the mechanism of proline and sugars is present in other categories of organisms such as fungi. There are even less bibliographic data on a relationship of the mechanism of proline and soluble sugars accumulation in fungi. The purpose of this study is to investigate proline and soluble sugars accumulation in the fungus Aspergillus creber under salt stress tolerance.