Background: Adolescence is the period of transition from children becoming adults that is marked by the occurrence of puberty, namely menstruation. At that time there was a change in the reproductive system that allows the occurrence of disturbances, one of which is menstrual cycle disorders.

Aim: Based on data from RISKESDAS (basic health survei) (2013), it shows that the percentage of menstrual cycle irregularities at the age of 10-29 years is 16.4% (RISKESDAS (basic health survei). Irregular menstrual cycles in adolescent girls aged 15-19 years are 11.7% and as many as 14.9% women who live in urban areas in Indonesia.

Methods: Samples were taken by using purposive sampling technique; it is obtained a sample of 35 students from grade 11 and 12 aged 16-18 years at SMAIT-Al-Ichwan Cikarang Utara. Data on the age at menarche and menstrual cycle with the method of interview used a questionnaire, food intake was obtained from 2x24 hours food recall form that is not in a row and hemoglobin status used Easy Touch Blood Hb tool conducted by nurses. Physical activity data was obtained by filling out a form and the results were calculated by using the PAR/PAL method. Statistical analysis used a chi-square test and used a fisher exact test value.

Results: The results of the reserach showed that there is no significant relationship between the intake of iron, folic acid, vitamin C and physical activity on menstrual cycle (p > 0.05).

Conclusion: This conclusion constitues there is a significant relationship between hemoglobin status on menstrual cycle (p = 0.005).