The findings of research proceedings on pesticide metabolites detection are both an environmental inventory and prevention of potential foodstuffs contaminants. Since, the requirement of a quality environment is a condition for ensuring food security, the newly designed methods with validated sensitivities and efficiencies should be highlighted as solutions for reducing pesticide metabolites. In this respect, this review provides information about pesticides metabolism subject to various analytical conditions applied to standard methods, in addition to the pesticide/related metabolites ratio (R) developed for the intended purpose.