The use of nanoparticles for the therapeutic purpose is gaining pronounced importance. In the last two decades, a number of nanomedicines received regulatory approval and several showed promises through clinical trials. In this content, it is important to synthesize nanoparticles from various sources and to check its efficiency, especially its antibacterial activity. In today’s scenario number nanomedicines are proving useful to control multidrug resistance and since the mechanism of action of nanoparticles is totally different from the small molecules like antibiotics it obviates the chances of drug resistance. In this review, we discussed three metal-based nanoparticles prepared from various reducing sources namely Zinc Oxide Nanoparticle (ZnO NPs), Titanium Dioxide Nanoparticle (TiO2 NPs) and Magnesium Dioxide Nanoparticle (MnO2 NPs). The focus also made towards the safety assessment of the several nanoparticles. In addition, the exact interaction of the nanoparticles with the bacterial cell surface and the resultant changes also been highlighted. The review put forward the sources, method, and antibacterial success of these nanoparticles so that future nanomedicines could be put forward.