Donation of the tissue or organ of human body from a living or dead person to a living recipient in need of transplantation is organ donation. In the field of modern medicine organ transplantation is one of the greatest scientific advances and remains the most challenging and complex. It saves thousands of life. The main objective of the study was to explore the knowledge and attitude of organ donation. A descriptive cross- sectional study using self- administered questionnaire tool was conducted among 154 Bachelor level students who were selected by using non probability consecutive sampling technique. The obtained data was entered on SPSS 20 version program and analyzed and interpreted by using descriptive statistics (Frequency, percentage, mean, median, and standard deviation) and inferential statistics (chi square).The study found that 57.1 percent had moderate knowledge on organ donation and more than half (69.5%) had neutral attitude on organ donation. Knowledge on organ donation was poor. There was significant association (0.014) between ethnicity and knowledge level. The study concluded that there was moderate level of knowledge and neutral level of attitude on organ donation among bachelor level students and there was no significant association of socio-demographic variables and level of knowledge except ethnicity. It therefore suggests awareness programs on organ donation for college students to promote and upgrade their knowledge and attitude about organ donation.