Mushrooms are generous source of nutritional, medicinal compounds, and industrial uses of the mushrooms still need to be worked out for their important applications. Mushrooms have significant enzymatic machinery allowing their application in different industries. Capabilities of different members of mushrooms have encouraged researchers to investigate further applications of these macrofungi in fields other than food and pharmaceutical industries. Specially, owing to the current shortage in global resources, contamination caused by plastic components and the incredible increase in population worldwide needs alternatives through macrofungi. Therefore this review aims to put light spot on enzymes production by mushrooms.