Incorporation of enzyme immobilized ZnO nanoparticles in to hydrophilic polymer Polymethyl metharcylate (PMMA) has been considered in this present study with the goal of developing antimicrobial hydrophobic textile coatings. The enzyme immobilized nanoparticles polymer coatings on textiles by dip coating, using the polymer Polymethyl methacrylate and proteolytic enzymes alpha chymotripsin immobilized green synthesized, amino functionalized ZnO nanoparticles. Immobilization of enzymes on nanoparticles with large surface to volume ratio provides obvious advantage. Alpha chymotripsin was immobilized on amino functionalized ZnO nanoparticles dispersed in n-proponal. The enzyme immobilized ZnO nanoparticles were used for making the ZnO/PMMA polymer nanocomposite coating. This coating was applied on cotton textile by dip coating and the coated textiles were evaluated for antimicrobial and hydrophobicity Properties.