The Central Sterile Supply Department (CSSD) is one of the hospital's critical support services. It is a place to receive medical equipment blood-stained from the other departments to be cleaned. It is responsible for cleaning, receiving, storing, sterilizing, and returning medical equipment to everyone and delivered to different departments within the hospital. Nowadays, various cleaning and dissolving blood stains are used to clean and remove blood stains. This research aimed to compare the efficacy of pre-cleaning spray and pre-cleaning spray foam cleaners for blood-contaminated surgical instruments and medical devices in the Central Sterile Services Department. The pre-cleaning spray was significantly higher than the pre-cleaning spray foam (p ≤ .005). A significant difference in the 2-minute pre-cleaning spray was observed by using cleaning indicators. The pre-cleaning spray foam started dissolving blood after 5 minutes. Therefore, from the study, using the pre-cleaning spray for dissolving blood stains and pre-cleaning has a more practical effect than pre-cleaning foam.