Irritable Bowel Syndrome (IBS) is a chronic disorder of the gastrointestinal system characterized by abdominal pain or discomfort and associated with different comorbidities like fibromyalgia, chronic pelvic pain, gastroesophageal reflux disease, anxiety and depression. In this study, the effects of COLPLATIR®, a nutraceutical product with a patented technology named BUTIRESOLV®, were investigated. In vivo and Ex-vivo analysis were carried out on IBS with diarrhoea (IBS-D) mouse model by intracolonic administration of Oil of Mustard (OM). Positive results have been obtained, thus demonstrating that the nutraceutical COLPLATIR® could reduce the abnormalities in intestinal motility, as observed in IBS.