Many mushroom genera are famous for their promising therapeutic capabilities. One of the mushrooms genera attracting attention is Cordyceps which has long been used in Asian countries for maintaining long and healthy life. Numerous studies on different metabolic activities of Cordyceps have been performed both in vitro and in vivo. Numerous studies on different metabolic activities of Cordyceps have been performed both in vitro and in vivo. However, it is debatable whether Cordyceps is a food supplement for health maintenance or a therapeutic "drug" carrying medicinal properties. The Cordyceps industry has developed greatly and offers thousands of products, commonly available in a global marketplace. This review describes the importance and the current status of the world Cordyceps cultivation, a range of products and industry practices along with suggestions for facilitating further research.
Mushrooms are macrofungi that have spore-bearing structures (fruiting bodies) and can be seen on soil surface as saprophytes, or growing as a parasite over their host. Mushrooms exist in two fungal phyla Basidiomycota and Ascomycota, and over 14,000 species are identified [1-5]. Mushrooms have well known nutritional value that can be compared to that of many vegetables, and act as substitute for meat [6-10]. Also, the saprophytic nature of mushrooms has contributed in the recycling capabilities of mushrooms which allow them to convert agro-wastes, food remains, and dead bodies to highly nutritious food [11-15]. On the other hand, mushrooms are rich sources of different compounds such as terpenes, phenolic compounds, fatty acids, α- and β-glucans, polysaccharides, proteins. Such compounds are responsible for the biological activities reported for many mushroom species including having anticancer, antiviral, antitumor, antidiabetic, antidepressive, antioxidant, immunomodulatory, anti-inflammatory, neuroprotective, hepatoprotective, nephroprotective, osteoprotective, hypotensive, antiallergic, antimicrobial, antihyperlipidemic, and hypocholesterolemic activity [16-20]. Previously, majority of studies have focused on certain species, especially those traditionally used among Asian culture [21-25]. However, there is a recent attention for the exploring mushrooms chemical composition in order to investigate the potential medical and pharmaceutical uses of these generous rich macrofungi [26-32].
The name Cordyceps comes from the Latin words cord which means ‘club’, and ceps, referring to ‘head’. The fruiting bodies of these fungi often erupts from the head of the larva and adult stages of many different species of insect. Cordyceps are entomophagous fungi from the phylum Ascomycota, family Ophiocordycipitaceae, order Hypocreales, and they are known to parasitize many orders of insects at different life stages from larva to adult stages. Numerous species within the genus have a golden reputation due to their long safe history of use in traditional medicines. Used for over 2000 years in China for treating infectious diseases, Cordyceps are also described in the ancient books of Ben-Cao-Cong-Xin (New Compilation of Materia Medica) which is as old as 1757 years AD, and ‘Ben Cao Gang Mu Shi Yi’ written by Xueming Zhao in 1765 AD. The Cordyceps genus contains some of the most highly prized and revered of all medicinal fungi. In spite of having a global distribution, the majority species of Cordyceps have been described from Asia. Grasslands, providing habitat for Thitarodes ghost moths and thus for Cordyceps sinensis, are a particularly important habitat and consists predominantly of sedges (Kobresia sp.), covering up to 80-90% of the subalpine grasslands [33]. The most famous and widely used species of Cordyceps is C. sinensis (Berk.) Sacc (syn. OphioCordyceps sinensis (Berk.) Sung, Sung, and Hywel-Jones & Spatafora.), and was also described as Sphaeria sinensis, then later renamed by Saccardo in 1878 as Cordyceps sinensis. Nowadays, the preferred scientific name is Cordyceps sinensis (Berk.) Sacc. The host range of this species is wide, including different species of Lepidopteran larvae, numerous species of Thitarodes caterpillars, and its most common host, the Himalayan bat moth Hepialus armoricanus. A similar species, C. militaris or as commonly known, the orange caterpillar fungus has a similar chemical composition and medicinal biological activities as C. sinensis [34].
Generally, Cordyceps species feed on insect larvae and sometimes they also parasite on mature insects. Cordyceps grow on all groups of insect-crickets, cockroaches, bees, centipedes, black beetles, and ants, to name a few. Although there are several species known to have medical value, only a few are cultivated and the most popular and well known are Cordyceps sinensis and Cordyceps militaris. However, Cordyceps, are not limited to insects and may grow on other arthropods as well as the fungi Elaphomyces Nees. This group belongs to the order Hypocreales, which includes 912 known species that are assigned to the families Cordycipitaceae and Ophiocordycipitaceae and partial Clavicipitaceae [35]. Cordyceps only refers to the macrofungi, and these macrofungi were previously placed in the old genus Cordyceps Fr. (Clavicipitaceae, Clavicipitales). Due to their special edible and medicinal values, Cordyceps is very popular in China, where a huge domestic market exists. By most Chinese people’s standards, Cordyceps only refers to ‘Dongchong Xiacao (worm in winter, herb in summer; O. sinensis, which is the most expensive type and only produced from the Tibetan Plateau; other Cordyceps species in the marketplace are termed ‘fake Dongchong Xiacao’, some of which may not be consumed in this way.
Cordyceps have a wide range of various compounds, some are characterized as nutritional compounds, since they possess all of the important amino acids, vitamins such as K and E, besides the water-soluble B vitamins (B1, B2, and B12). In addition, they contain many sugars, including mono-, di-, and oligosaccharides, and many complex polysaccharides, proteins, sterols, nucleosides, and trace elements (Na, K, Ca, Mg, Al, Fe, Cu, V, Pi, Se, Ni, Sr, Si, Ti, Cr, Ga, Zn, and Zr) [9,15]. Cordyceps contains abundance of polysaccharides, which represents in the range of 3–8% of the overall weight, and commonly originated from the fruiting bodies. Cordyceps polysaccharide, is one of the main bioactive components along with nucleotides (including adenosine, uridine and guanosine). Investigations show that guanosine is the nucleotide in greatest abundance in both natural and cultivated Cordyceps [15]. Cordyceps sinensis is the most expensive and the most extensively studied Cordyceps species. C. sinensis contains crude fats, proteins, fiber, carbohydrate, cordycepin (30-deoxyadenosine), cordycepic acid (D-mannitol), polysaccharide and a series of vitamins. The therapeutic applications of Cordyceps are focusing mostly on the major effects of increasing utilization of oxygen and production of ATP, besides stabilizing sugar metabolism in blood. Such activities may be attributed to compounds as cordycepin, cordycepic acid and numerous vitamins, polysaccharides and trace elements. Although all the medically active compounds of C. sinensis are still unknown, at least two chemical compounds, cordycepin and cordycepic acid, have been purified and identified as medically important active compounds. It is now believed that cordycepic acid is, in fact, D-mannitol, and that cordycepin is 30-deoxyadenosine (30-dA), a purine alkaloid. Of all Cordyceps species, Cordyceps militaris has been most successfully cultivated and most intensively studied. Most Cordyceps products in the marketplace are developed from the fruiting bodies of cultivated C. militaris. According to chemical analysis, C. militaris contains cordycepin, Adenosine, Polysaccharide, Mannitol, Trehalose, Polyunsaturated fatty acids, δ-tocopherol, p-Hydroxybenzoic acid and β-(1→3)-D-glucan [36,37].
The natural fruiting bodies of Cordyceps are very rare and costly to collect. Moreover, natural populations of key Cordyceps species are decreasing rapidly due to over collection [38], presenting the need increased cultivation of Cordyceps in vitro using an artificial medium. The percentage of species that been successfully cultivated in artificial media to the total identified Cordyceps species is very low. Examples of some medicinally important Cordyceps species such as Cordyceps sinensis, artificial O. sinensis, Cordyceps militaris, and artificial Cordyceps militaris. Strain CS-4 (Paecilomyces hepiali Chen.) was isolated as early as 1982 as one of the first commercially used strains of Cordyceps. After a lot of clinical trials, the chemical composition, biological activity and toxicity of this strain became well known. The first large scale fruiting techniques used for growing Cordyceps reduced the natural growing cycle from 5 to 2 years, this technique included breeding the host larvae, Thitarodes (Hepialus), then placing about 100 larvae into shoe carton-sized plastic containers covered with lids, which are filled with grassland soil comprising tubers and roots originated from their natural foods, as well as other roots from cultivation. The C. sinensis spores are inoculated after two years and about 10% of the larvae are actually taken over by Cordyceps and grow stromata [39]. On the other hand, Arora, et al. [40], succeeded in using submerged conditions for culturing Cordyceps sinensis at pH 6 and temperature 15ºC. The growth of C. sinensis on sabouraud’s dextrose with yeast extract broth medium was also investigated using different carbon sources, nitrogen sources, and additives (vitamins and minerals) [41]. The greatest number of conidia were obtained under the physical stress of freeze-shock. Sucrose was the best carbon source for C. sinensis growth while Beef extract and yeast extract were the best nitrogen sources [42]. Moreover, using folic acid significantly increased the yield, and adding calcium chloride and zinc chloride as micro and macronutrients, respectively increased the total yield significantly.
One of the remarkably important artificial techniques for C. sinensis culturing was using sterile rice media at 9-13°C for 40-60 days followed by lowering temperature to 4°C for inducing stroma production and at 13°C for 40 days for the process of developing the fruiting bodies. It should be mentioned that the Cordyceps mycelium growth depends on different factors such as growth media, temperature, pH, and some environmental factors, but after trying different media, potato dextrose agar was proven to be the best medium using a pH range of 8.5-9.5 at 20- 25°C [43]. Complete artificial cultivation is achieved by inoculating reared larvae with cultured strains and the infected larvae were monitored and fed indoors for one or two years. After that, C. sinensis could be collected. On the contrary, in semi-natural cultivation, the natural habitats was used to allow infected larvae to grow freely for 3-5 years, then C sinensis could be collected from the released areas. Cordyceps militaris cultivation is much easier than C. sinensis in both solid and broth media using numerous carbon and nitrogen sources, since C. militaris can complete its whole life cycle when cultivated in-vitro [44]. Cultivation of C. militaris mycelium using artificial media has lately been developed specially for the purpose of Cordycepin production using different methods such as surface culture and submerged culture. Generally, C. militaris Stromata production requires 35-70 days. Nevertheless, culturing duration is critically affected by various conditions such as medium amount, volume and shape of the container used in culturing process. The growth of C. militaris stroma cultivation in vitro started with using insects to grow stromata of C. militaris followed by laboratory trials using various organic substrates. Cereals such as rice have been commonly used with some organic substrates for commercial production of C. militaris stromata [45]. Other successful substrates include cottonseed coats, wheat grains, bean powder, corn grain, corn cobs, millet, and sorghum. The optimum organic substrate currently used is a mixture of rice and silkworm pupae. Additionally, studies have reported malt, brown rice, and soybean as superior nutritional sources for C. militaris in comparison with chemical media. C. militaris cultivation requires a relatively low level of nitrogen, which may explain lower yields when using insects in comparison with higher yields achieved when cereals were used in the culture. Plant hormones such as colchicines, 2, 4-D, citric acid triamine can promote C. militaris stroma production [46]. Additionally, potassium, calcium, and magnesium salts at a concentration of 0.1 g/l can increase the yield of fruiting bodies. Mycelia production for the purpose of biologically active compounds production is also possible and has been conducted in submerged culture. C. militaris cultivation has been further advanced, resulting in a high yield of stromata production and high content of Cordycepin. Furthermore, the fruiting bodies production has been investigated using multi-ascospore isolates and their progeny strains for three successive generations and it was found that F1 progeny strains produced a higher number of fruiting bodies [47]. Also using different media [48].
Species of Cordyceps are widely researched due to the endless list of medicinal biological activities exerted by their extracted compounds as shown by some examples in table 1, with various medical and nutritional values. The main uses of Cordyceps have been known in oriental old medicine for curing respiratory diseases such as asthma and bronchial cases, as well as for providing body with energy and for boosting sexual power. Modern research now confirms the efficiency of Cordyceps in many other fields. One of the breakthroughs of modern research has been the discovery of cordycepin, which has a strong antimicrobial activity against almost all species of bacteria exhibiting resistance to frequently used antibiotics. Cordyceps showed strong activity against tuberculosis, leprosy and human leukaemia, as shown in many clinical trials in Asia and elsewhere. Cordyceps was shown to be potent in increasing the maximum amount of oxygen and to improve respiratory function. There are a number of components like deoxynucleosides produced by Cordyceps sinensis, such as the compounds 2’, 3’ deoxyadenosine which is marketed under the trade name "Didanosine" in the USA as a medication for treatment of AIDS. Similarly, Quinic acid derived from Cordycepin (3’ deoxyadenosine) present in Cordyceps is found to have antiviral and antibacterial property [49]. Numerous studies have verified the benefits of C. sinensis in treating disturbances in heart rhythm as cardiac arrhythmia and chronic heart failure.
Table 1: Common therapeutic effects of different Cordyceps sp. | ||
Therapeutic effects | Cordycepsspp. | Major bioactive compounds |
Antitumor | C. sinensis | Cordycepin Cordyglucans Monosaccharide saponins EPSF |
C. militaris | cordycepin and mannitol | |
Anti-diabetic effects | C. sinensis C. militaris |
Cordymin Cordycepin, adenosine |
Anti-inflammatory | C. sinensis C. militaris | Cordycepin Adenosine β-(1®3)-D-glucan |
Anti-oxidant activity | C. sinensis | Exopolysaccharide fraction, EPSF CPS-1 CME-1 |
C. militaris | Polysaccharide (PSC) | |
Antimicrobial activity | C. sinensis C. militaris | Cordycepin Ergosterol Mannitol, trehalose, Polyunsaturated fatty acids, δ-tocopherol and p-Hydroxybenzoic acid |
Anti-influenza | C. militaris | Polysaccharide (PSC) |
Anticonvulsant activity | C. sinensis | Adenosine |
Various biologically active compounds exerting an anticancer activity were extracted from Cordyceps. Cordycepin has an antitumor activity in B16 melanoma cells. Cordycepin induced apoptosis in Mouse Leydig tumor cell in vitro. Also, it inhibits cell proliferation and further apoptosis of human colorectal carcinoma using SW480 and SW620 in vitro. In gallbladder cancer cell, cordycepin causes loss of cancer cell viability and apoptosis via inhibiting the mammalian target of rapamycin complex 1 [50]. C. militaris was found to inhibit U937 cells grown in a dose dependent manner and also in the treatment of human leukaemia. Cordyceps has shown promising activities in inhibiting the growth of cancer cells [51] and in some cases could reduce tumor size. Clinical trials on cancer patients have been conducted in many Asian countries, showing promising results in reducing tumor size, improving tolerance for chemotherapy and/or radiation and in stimulating the immune system which hence enhances the efficiency of chemotherapy. Ethanolic extract of C. militaris showed a potent antitumor effect in RMA cell-derived tumors at a xenograft mouse model [52]. Moreover, some Cordyceps species have anti-leukaemia activities and ameliorate suppressive effects of chemotherapy on bone marrow function as a model for cancer treatment [53].
Cordyceps are found to regulate and also lower the blood sugar levels by improving metabolism of glucose and conserving hepatic glycogen [3]. Furthermore, Cordyceps can increase secretion of glucokinase and hexokinase which are glucose regulating enzymes secreted by the liver [54]. Polysaccharides are the key player in showing the hypoglycaemic activity of Cordyceps. For example, the polysaccharide (CS-F30) obtained from C. sinensis culture mycelium exerted a potent hypoglycaemic activity when intraperitoneal administrated in genetically diabetic mice. In addition, dramatic reduction in plasma glucose level was reported after intravenous administration of CS-F30 in normal and streptozocin-induced diabetic mice. A different polysaccharide, (CS-F10), was extracted and identified from a hot-water extract of C. sinensis cultured mycelia and composed of galactose, glucose and mannose in a molar ratio of 43:33:24, could successfully lower the level of plasma glucose in normal, adrenaline-induced hyperglycaemic and diabetic mice. Hypercholesterolemia is an indicator for high risk of cardiovascular attack. Many studies have reported the role of C. sinensis in lowering the total cholesterol level and the level of triglycerides (Geng 1985). It also helps in increasing the ratio of the good cholesterol [High Density Lipoprotein (HDL cholesterol)] to bad cholesterol [Low Density Lipoprotein (LDL cholesterol)]. A hot-water extract of the mycelia of C. sinensis has been proven to decrease serum total cholesterol concentration in tested mice, by reducing LDL and very-low-density lipoprotein, and elevating the concentration of the good cholesterol (HDL cholesterol) [55].
The results of some clinical trials revealed that the administration of C. sinensis could significantly improve kidney function and overall immunity of patients suffering from chronic renal failure. Moreover treating patients with gentamicin induced kidney damage helped in recovering 89% of normal kidney function in a relatively short time. The mechanism of kidney enhancing activity of Cordyceps is owing to its capability to elevate 17- ketosteroid and 17-hydroxycorticosteroid levels in the body, protect sodium pump activity of tubular cells, accelerate tubular cells regeneration, and reduce calcium content in certain tissues. Cordyceps is universally involved as co- treatment of chronic hepatitis B and C. Extract mixture of Cordyceps in combination with other medicinal mushrooms in addition to the antiviral drug, lamivudine, was used for treating hepatitis B [56]. On the other hand, daily consume of Cordyceps improved liver functions in patients suffering from post-hepatic cirrhosis.
Cordyceps has been used from centuries as a remedy for weakness and fatigue by residents living in the high mountains of Tibet to give them energy which is achieved by increasing cellular ATP [57]. Nowadays, Cordyceps is used by athletes to fight fatigue and weakness and to increase endurance and improve energy levels. Additionally, clinical trials involving elderly patients with chronic fatigue, results indicated that treatment with C. sinensis resulted in improvement of fatigue and dizziness, increasing cold intolerance, and amnesia [58].
Cordyceps sinensis also has obvious effects on other organ systems. For example, in the central nervous system, C. sinensis has cooling, anticonvulsant and sedative activities. For the respiratory, C. sinensis has a strong relaxant activity on the bronchi, considerably, and also plays a key role in contraction of trachea caused by histamine, it as well has an antitussive, expectorant and anti-asthmatic effect and prevents pulmonary emphysema. Concerning endocrine system, C. sinensis increases secretion of adrenaline, has effects as a male hormone; Polysaccharides extracted from Cordyceps can increase corticosterone level in plasma. Cordyceps is used in traditional medicine for decades to improve men fertility. A study has proven the positive effect of using C. militaris mycelium on sperm motility, morphology, productivity, and enhancement of sexual activity, Cordyceps extracts contain adenosine, deoxyadenosine, related adenosine type nucleotides, and nucleosides which help in stabilizing heartbeat and correcting heart arrhythmias. Such compounds have an effect on coronary and cerebral circulation. Cordyceps is known to be used in treatment of chronic hepatitis B and C. Moreover, consuming Cordyceps resulted in improving liver function tests in patients suffering from post-hepatic cirrhosis [3].
Generally, cordycepin is the metabolite responsible for the anti-inflammatory activity of many Cordyceps species. Ethanolic extracts of cultured mycelia and fruiting bodies of C. militaris exhibited an anti-inflammatory effect on chorioallantoic membrane angiogenesis of chick embryo, similarly in the croton oil-induced ear edema in mice. On the other hand, an alkaline extract of C. militaris, showed a potent anti-inflammatory effect against formalin-induced nociception and LPS-induced peritonitis in mice, due to containing a potent anti-inflammatory compound (linear b- (1R3)-D-glucan) [37]. The strain CBG-CS-2 (Paecilomyces hepiali of the Cordyceps spp.), can be used for its modulatory effects on macrophages inflammatory system as an anti-inflammatory supplement [59]. A butanol fraction from C. bassiana exhibited an anti-inflammation activity in LPS-stimulated RAW 264.7 macrophages [60,61]. Adenosine is another compound existing in Cordyceps species with a wide spectrum of activities related to preventing tissue damage as anti-inflammatory properties. It’s worth noting that the content and number of adenosines existing in cultured C. sinensis is higher than that in the natural one. The methanolic fraction of C. militaris fruiting bodies exerted an anti-inflammatory activity resulting from the presence of cordycerebroside A, soyacerebroside I, and glucocerebroside which prevented accumulation of the pro-inflammatory iNOS protein and decreased the expression of the COX-2 protein in LPS-stimulated RAW264.7 macrophages [62].
Protecting against damage of cells by free radicals is one of the biological activities exerted by Cordyceps species extracts. This activity is corresponding to polysaccharide fraction. C. sinensis has potent antioxidant and anti-ageing properties. Many studies elucidated the antioxidant effect of extracts obtained from C. militaris. The fruiting bodies extract of C. militaris had a potent DPPH· radical scavenging activity, whereas the fermented extract of mycelia had stronger total antioxidant activity, and reducing ability [63].
Cordyceps is generally safe in recommended dosage and no major side effects were reported [64].
The Cordyceps industry is strong and growing. Various products were commercialized for compounds originated from Cordyceps species. Some major Cordyceps based companies are listed in table 2, and examples for some cosmetics containing Cordyceps sinensis and Cordyceps militaris extracts and their beneficial functions are mentioned in table 3. The decline in wild-harvest, compounded by ever increasing global demand is leading to record prices and global attention [65]. Global production of just O. sinensis is estimated to be in the region of 85 to 185 tons, with further tonnage provided by other Cordyceps species. The harvesting and sale of non-cultivated Cordyceps can have a significant impact on household incomes in the regions in which it is collected. For example, a survey in 2004 of rural Tibet revealed that 40% of annual household cash income and 8.5% of GDP was generated by sales of O. sinensis [66]. This sizable contribution to the Tibetan economy is reflected in the fact that global prices have risen dramatically at an average rate of 21.2% per annum, inflation adjusted, between 1997 and 2004. This high value product is an economic boon to rural areas but this comes at a cost. For example, conflicts over collecting-grounds may be common, causing difficulties for local governing bodies and occasionally resulting in violence, such as the death of seven farmers in 2009. A dramatic rise in harvesting intensity has also been implicated in a possible collapse of this species in some areas, such as rural china where a few thousand kgs were collected 2001, representing a decrease of over 70%. Further, associated conservational issues with high intensity collection in these sensitive grassland ecosystems on flora and fauna can be significant [67]. Indeed, the flora that is being impacted may itself be important for O. sinensis survival, further contributing to a decline [68]. Clearly continued wild-harvesting of Cordyceps at the current rate may not be sustainable and this is where the burgeoning cultivation industry may be able to contribute.
Table 2: List of major Cordyceps based companies. | |
Cordyceps Company | Country of the origin |
ALOHA MEDICINALS | USA https://www.alohamedicinals.com |
DOCTORS BEST | USA https://www.drbvitamins.com |
HOSTDEFENSE MUSHROOMS | USA https://hostdefense.com |
PERFECTSUPPLEMENTS | USA https://www.perfectsupplements.com |
PARADISE | USA https://paradiseherbs.com |
SOLARAY | USA https://www.naturalhealthyconcepts.com |
OREGONSWILDHARVEST | USA https://www.oregonswildharvest.com |
REALHERBS | USA http://www.irealherbs.com |
MUSHROOMSCIENCE | USA https://mushroomscience.com |
HERBSENS | CHINA https://www.herbsens.com |
TEREZIA | CZECH https://www.terezia.eu |
ZEINPHARMA | Germany https://www.zeinpharma.com |
THE REALLY HEALTHY | UK https://www.healthy.co.uk |
Table 3: Cosmetic products containing Cordyceps sinensis and Cordyceps militaris extract and their functions. | ||
Product Name | Function | References |
Cordyceps (MUSHROOM EXTRACT) | Improved lungs and kidney function | https://organika.com |
CORDYCEP | Support healthy immune and vascular systems | https://moonjuice.com |
ULTRA Cordyceps PLUS | Support lung health and liver function | https://www.drbvitamins.com |
HOST DEFENSE Cordyceps | Promotes healthy kidney function and Augments oxygen uptake | https://hostdefense.com |
PERFECT Cordyceps | Boost the Immune System and Improve Sexual Function | http://www.perfect-supplements.com |
PARADISE HERBS TIBETAN Cordyceps | Support physical activity, performance, stamina and resistance | https://paradiseherbs.com |
SOLARAY Cordyceps | Protect against throat infections and promotes healthy cholesterol levels | https://www.naturalhealthyconcepts.com |
OREGON'S WILD HARVEST Cordyceps | Cardiovascular, respiratory and immune support | https://www.oregonswildharvest.com |
Cordyceps | Boosts energy and immunity and supports cardiovascular health | http://www.irealherbs.com |
Cordyceps CS-4 | Provide the immune health benefits | https://mushroomscience.com |
Cultivation of currently exploited as well as new species, is an emerging trend in the Cordyceps industry, with new isolates frequently being reported. However, despite the recent successes in cultivation, there remains many challenges including (1) how to reduce the rearing cost of the ghost moth; (2) how to prevent contaminating microbes during the cultivation cycle; (3) how to streamline the cultivation process [69]. Cordyceps cultivation remains an area of intense activity and developments are expected that may aid the economic and reliable cultivation of these species. The intense global interest and value assigned to Cordyceps has led to a large range of commercial products derived from these fungi all over the world as shown in figures 1-3. In parallel, there has emerged an issue with counterfeit and contaminated products [70]. Although this is not an issue confined to Cordyceps products, increased quality control and authentication methods are needed and these are being developed using, for example, nucleosides, ergosterol, mannitol and polysaccharides markers. Despite the challenges in cultivation, harvesting and downstream products, top quality Cordyceps trade for around 100,000 USD/kg [65] a clear sign that the industry is strong and here to stay.
The authors declare no conflict of interest.
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